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PMID: 166378 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 72 ·No. 6 ·1975-06-00 ·Pages 2064-8

Gill DM

Abstract

NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.

MeSH Terms
Adenylyl Cyclases/metabolism Animals Columbidae Enzyme Activation Erythrocytes/drug effects,enzymology,metabolism Hydrolysis N-Glycosyl Hydrolases/pharmacology NAD/analogs & derivatives,metabolism,pharmacology Neurospora/enzymology Rabbits Species Specificity Structure-Activity Relationship Toxins, Biological/pharmacology Turkeys Vibrio cholerae
Chemicals
Toxins, Biological NAD N-Glycosyl Hydrolases Adenylyl Cyclases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Gill D M
References (4)
4 references, click to expand
  1. [DPN- and TPN-specific nucleosidases in erythrocytes].
    Biochim Biophys Acta. 1955 Oct;18(2):296 PMID: 13276383
  2. Studies on the mode of action of diphtheria toxin. IV. Specificity of the cofactor (NAD) requirement for toxin action in cell-free systems.
    J Exp Med. 1967 Nov 1;126(5):913-21 PMID: 4294108
  3. Determination of subpicomole levels of NADH and FMN using bacterial luciferase and the liquid scintillation spectrometer.
    Anal Biochem. 1971 Feb;39(2):441-53 PMID: 4324531
  4. Poly (adenosine diphosphate ribose) synthesis in soluble extracts of animal organs.
    J Biol Chem. 1972 Sep 25;247(18):5964-71 PMID: 4341497
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.72.6.2064
Published
1975-06-00
Pages
2064-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC432694
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.72.6.2064 PMC Full Text View journal details
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