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PMID: 16640457 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Linking splicing to Pol II transcription stabilizes pre-mRNAs and influences splicing patterns.

PLoS biology ·Vol. 4 ·No. 6 ·2006-06-00 ·Pages e147

Hicks MJ, Yang CR, Kotlajich MV, Hertel KJ

Abstract

RNA processing is carried out in close proximity to the site of transcription, suggesting a regulatory link between transcription and pre-mRNA splicing. Using an in vitro transcription/splicing assay, we demonstrate that an association of RNA polymerase II (Pol II) transcription and pre-mRNA splicing is required for efficient gene expression. Pol II-synthesized RNAs containing functional splice sites are protected from nuclear degradation, presumably because the local concentration of the splicing machinery is sufficiently high to ensure its association over interactions with nucleases. Furthermore, the process of transcription influences alternative splicing of newly synthesized pre-mRNAs. Because other RNA polymerases do not provide similar protection from nucleases, and their RNA products display altered splicing patterns, the link between transcription and RNA processing is RNA Pol II-specific. We propose that the connection between transcription by Pol II and pre-mRNA splicing guarantees an extended half-life and proper processing of nascent pre-mRNAs.

MeSH Terms
DNA-Directed RNA Polymerases/physiology Globins/genetics HeLa Cells Humans Kinetics Models, Genetic Plasmids/genetics RNA Polymerase II/metabolism RNA Precursors/metabolism RNA Splice Sites/physiology RNA Splicing/physiology RNA Stability RNA, Messenger/metabolism Spliceosomes/physiology Transcription, Genetic Viral Proteins/physiology
Chemicals
RNA Precursors RNA Splice Sites RNA, Messenger Viral Proteins Globins RNA Polymerase II bacteriophage T7 RNA polymerase DNA-Directed RNA Polymerases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Hicks Martin J
Department of Microbiology and Molecular Genetics, University of California Irvine, Irvine, California, USA.
Yang Chin-Rang
Kotlajich Matthew V
Hertel Klemens J
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Article Info
Journal
PLoS biology
Abbr.
PLoS Biol
ISSN
1545-7885
DOI
10.1371/journal.pbio.0040147
Published
2006-06-00
Epub
2006-00-02
Pages
e147
Language
English
Region
United States
NLM ID
101183755
PMCID
PMC1450099
Subset
IM
Grants
NIGMS NIH HHS · R01 GM062287 · United States
NIGMS NIH HHS · GM 68093 · United States
NIGMS NIH HHS · GM 62287 · United States
NLM NIH HHS · T15LM 07443 · United States
NLM NIH HHS · T15 LM007443 · United States
Corrections
CommentIn
PMID: 20076584
External Links
PubMed source DOI: 10.1371/journal.pbio.0040147 PMC Full Text View journal details
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