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PMID: 16656637 Published · ppublish English Journal Article

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris. I. Localization of Activity in the Chloroplasts of a Chloroplast Containing Fraction from Developing Leaves.

Plant physiology ·Vol. 42 ·No. 9 ·1967-09-00 ·Pages 1179-86

Parenti F, Margulies MM

Abstract

The incorporation of uniformly labeled leucine-(14)C into protein by a chloroplast containing fraction from developing primary leaves of bean is reported. Chloroplasts, obtained from week old plants grown in darkness, and then illuminated with white light for 12 hours, were shown to be the principal sites of incorporating activity. Incorporation may continue for 2 hours. Rates of up to 50 mumumole leucine incorporated per mg protein per hour are observed when a 1 hour assay period is used. Incorporation is only partially sensitive to ribonuclease.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Parenti F
Radiation Biology Laboratory, Smithsonian Institution, Washington, D. C. 20560.
Margulies M M
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21 references, click to expand
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.42.9.1179
Published
1967-09-00
Pages
1179-86
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1086699
External Links
PubMed source DOI: 10.1104/pp.42.9.1179 PMC Full Text View journal details
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