Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.
A technique is described for detecting mutants of Chlamydomonas reinhardi that have impaired photosynthesis. It is based on the fact that when photosynthetic electron transport is blocked there may be an increase in the level of fluorescence similar to that obtained in the presence of an inhibitor of photosynthetic electron transport such as dichlorophenyldimethylurea.
7 references, click to expand
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MUTATIONS OF UNICELLULAR GREEN ALGAE AND THEIR APPLICATION TO STUDIES ON THE MECHANISM OF PHOTOSYNTHESIS.Rec Chem Prog. 1964 Sep;25:181-95 PMID: 14211157
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Photosynthetic Electron Transport Chain of Chlamydomonas reinhardi VI. Electron Transport in Mutant Strains Lacking Either Cytochrome 553 or Plastocyanin.Plant Physiol. 1966 Dec;41(10):1648-56 PMID: 16656453
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THE PHOTOSYNTHETIC ELECTRON TRANSPORT CHAIN OF CHLAMYDOMONAS REINHARDI. I. TRIPHOSPHOPYRIDINE NUCLEOTIDE PHOTOREDUCTION IN WILD-TYPE AND MUTANT STRAINS.J Biol Chem. 1963 Dec;238:4052-7 PMID: 14086744
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A screening technique for photosynthetic mutants in unicellular algae.Nature. 1960 Oct 22;188:339-40 PMID: 13761501
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Cytochrome f and plastocyanin: their sequence in the photosynthetic electron transport chain of Chlamydomonas reinhardi.Proc Natl Acad Sci U S A. 1965 Dec;54(6):1665-9 PMID: 4379719
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Damage induced by methyl methanesulfonate (MMS) in Chlamydomonas reinhardi.Z Vererbungsl. 1966;98(3):193-202 PMID: 5987732
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