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PMID: 16657503 Published · ppublish English Journal Article

Porphyrin Biosynthesis in Cell-free Homogenates from Higher Plants.

Plant physiology ·Vol. 46 ·No. 4 ·1970-10-00 ·Pages 543-9

Rebeiz CA, Haidar MA, Yaghi M

Abstract

The porphyrin and phorbin biosynthetic activity of etiolated cucumber (Cucumis sativus, L.) cotyledons was compared to that of cotyledonary homogenates. Etiolated cotyledons incubated with delta-aminolevulinic acid accumulate protoporphyrin, coproporphyrin, small amounts of Mg protoporphyrin monoester, and trace amounts of uroporphyrin. They also incorporate 4-(14)C-delta-aminolevulinic acid into free porphyrins, protochlorophyllide, protochlorophyllide phytyl ester, and Mg protoporphyrin monoester. Homogenates incubated with delta-aminolevulinic acid likewise accumulate coproporphyrin, uroporphyrin, Mg coproporphyrin, and trace amounts of protoporphyrin. They also incorporate 4-(14)C-delta-aminolevulinic acid into Mg protoporphyrin monoester, Mg coproporphyrin, and free porphyrins. However, the capacity to synthesize protochlorophyllide and protochlorophyllide phytyl ester is lost and the endogenous protochlorophylls gradually disappear. Mg protoporphyrin monoester represents the terminal biosynthetic step in this cell-free system.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Rebeiz C A
Department of Biological Sciences, Scientific and Agricultural Research Institute, Fanar, Lebanon.
Haidar M A
Yaghi M
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.46.4.543
Published
1970-10-00
Pages
543-9
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC396633
External Links
PubMed source DOI: 10.1104/pp.46.4.543 PMC Full Text View journal details
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