Assay of adenosine 5-triphosphate sulfurylase by pyrophosphate exchange.

Plant physiology ·Vol. 47 ·No. 1 ·1971-01-00 ·Pages 114-8

Shaw WH, Anderson JW

Abstract

The sulfate-dependent pyrophosphate exchange reaction has been re-examined and confirmed. Standard assay conditions for measuring ATP sulfurylase by sulfate-dependent pyrophosphate exchange are described and some properties of the enzyme (measured in crude dialyzed extracts) are reported. This method has many advantages over the well established molybdate method.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Shaw W H

Botany Department, La Trobe University, Bundoora, Vic. 3083, Australia.

Anderson J W

References (6)

6 references, click to expand

Enzymatic reactions involving sulfate, sulfite, selenate, and molybdate.
J Biol Chem. 1958 Oct;233(4):975-81 PMID: 13587526
ATP Sulfurylase Activity in the Soybean [Glycine max (L.) Merr.].
Plant Physiol. 1968 Dec;43(12):2041-4 PMID: 16657005
Influence of age and sulfur metabolism on ATP sulfurylase activity in the soybean and a survey of selected species.
Plant Physiol. 1969 Sep;44(9):1241-6 PMID: 16657197
The isolation of a tryptophan-activating enzyme from pancreas.
Arch Biochem Biophys. 1956 Nov;65(1):21-38 PMID: 13373404
The estimation of phosphorus.
Biochem J. 1940 Jun;34(6):858-65 PMID: 16747230
Amino acid dependent exchange between pyrophosphate and adenosine triphosphate in spinach preparations.
J Biol Chem. 1959 May;234(5):1238-40 PMID: 13654354

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.47.1.114

Published

1971-01-00

Pages

114-8

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC365822

External Links

PubMed source DOI: 10.1104/pp.47.1.114 PMC Full Text View journal details

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Assay of adenosine 5-triphosphate sulfurylase by pyrophosphate exchange.

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