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PMID: 16658236 Published · ppublish English Journal Article

Synthesis and turnover of nitrate reductase in corn roots.

Plant physiology ·Vol. 50 ·No. 6 ·1972-12-00 ·Pages 649-54

Oaks A, Wallace W, Stevens D

Abstract

THE INDUCTION AND REINDUCTION OF NITRATE REDUCTASE IN ROOT TIP OR MATURE ROOT SECTIONS SHOW ESSENTIALLY A SIMILAR PATTERN: a lag, a period of rapid increase in enzyme activity and finally a period of relatively minor change. Both inductions are sensitive to 6-methylpurine and cycloheximide. Kinetic studies with 6-methylpurine suggest that the half-life of the messenger RNA for nitrate reductase in both sections is about 20 minutes. The rate of decay of nitrate reductase activity induced by transfer to a nitrate-free medium is slower in root tips (t(1/2) = 3 hours) than in mature root sections (t(1/2) = 2 hours). The enzyme from mature root sections is also less stable to mild heat treatments (27 C; 40 C) than the enzyme from root tip sections. The results indicate that factors regulating enzyme turnover show important changes as root cells mature and may be significant in determining steady state levels of the enzyme.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Oaks A
Department of Biology, McMaster University, Hamilton, Ontario, Canada.
Wallace W
Stevens D
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.50.6.649
Published
1972-12-00
Pages
649-54
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC366209
External Links
PubMed source DOI: 10.1104/pp.50.6.649 PMC Full Text View journal details
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