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PMID: 16658442 Published · ppublish English Journal Article

Immunochemistry of phytochrome.

Plant physiology ·Vol. 51 ·No. 5 ·1973-05-00 ·Pages 939-45

Rice HV, Briggs WR

Abstract

Rabbit antibody was elicited against purified oat phytochrome polypeptides. Immunodiffusion and immunoelectrophoresis indicated the antibody elicited was predominantly a single precipitin system. No antigenic difference was detected between red-absorbing phytochrome and far red-absorbing phytochrome. Crude preparations of rye and corn phytochrome showed a line of identity when cross-reacted with oat polypeptide phytochrome; pea phytochrome showed a line of partial identity. Precipitin reactions with purified rye phytochrome analyzed with sucrose density gradient centrifugation and immunodiffusion confirmed that the same class of determinants was available to the antibody when the protein was known to be in a state which had not undergone extensive proteolytic attack.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Rice H V
The Biological Laboratories, Harvard University, Cambridge, Massachusetts 02138.
Briggs W R
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.51.5.939
Published
1973-05-00
Pages
939-45
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC366378
External Links
PubMed source DOI: 10.1104/pp.51.5.939 PMC Full Text View journal details
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