Characterization of Inositol-containing Phosphosphingolipids from Tobacco Leaves: Isolation and Identification of Two Novel, Major Lipids: N-Acetylglucosamidoglucuronidoinositol Phosphorylceramide and Glucosamidoglucuronidoinositol Phosphorylceramide.

Plant physiology ·Vol. 55 ·No. 1 ·1975-01-00 ·Pages 120-9

Kaul K, Lester RL

Abstract

A method for a large scale extraction of phosphoglycosphingolipids from the leaves of Nicotiana tabacum L. has been developed. The phosphosphingolipid concentrate consists of a dozen or more polar lipids as judged by thin layer chromatography. Two of these lipids were purified by chromatography on porous silica beads and partially characterized. These lipids are formulated as: N-acetylglucosamidoglucuronidoinositol phosphorylceramide and glucosamidoglucuronidoinositol phosphorylceramide. Although not fully characterized, the other lipids in the concentrate are inositol-containing phosphosphingolipids with a higher carbohydrate content.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Kaul K

Department of Biochemistry, College of Medicine, University of Kentucky, Lexington, Kentucky 40506.

Lester R L

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.55.1.120

Published

1975-01-00

Pages

120-9

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC541565

External Links

PubMed source DOI: 10.1104/pp.55.1.120 PMC Full Text View journal details

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Characterization of Inositol-containing Phosphosphingolipids from Tobacco Leaves: Isolation and Identification of Two Novel, Major Lipids: N-Acetylglucosamidoglucuronidoinositol Phosphorylceramide and Glucosamidoglucuronidoinositol Phosphorylceramide.

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