Purification of NADH-Nitrate Reductase by Affinity Chromatography.

Plant physiology ·Vol. 56 ·No. 6 ·1975-12-00 ·Pages 853-5

Solomonson LP

Abstract

ASSIMILATORY NITRATE REDUCTASE (NADH: nitrate oxidoreductase, EC 1.6.6.1) from Chlorella vulgaris has been purified to electrophoretic homogeneity with an overall yield of 60% by a procedure that utilizes blue dextran-agarose as an affinity column. Nitrate reductase binds to blue dextran and is not eluted in the presence of high ionic strength buffer but is rapidly eluted in the presence of mumolar concentrations of NADH.

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Solomonson L P

Department of Biochemistry, University of Iowa, Iowa City, Iowa 52242.

References (6)

6 references, click to expand

Further purification and properties of Neurospora nitrate reductase.
J Biol Chem. 1969 Jun 10;244(11):2870-82 PMID: 4388959
Activation of nitrate reductase by oxidation.
Biochim Biophys Acta. 1972 Aug 17;275(2):276-8 PMID: 5077863
Properties of a nitrate reductase of Chlorella.
Biochim Biophys Acta. 1972 Jun 23;267(3):544-57 PMID: 4340061
Rapid purification of lactate dehydrogenase from rat liver and hepatoma: a new approach.
Arch Biochem Biophys. 1974 Jan;160(1):279-84 PMID: 4364067
[Reciprocity of yeast-phosphofructokinase with dextran blue 2000].
Eur J Biochem. 1971 Sep 13;22(1):40-5 PMID: 4255612
Blue dextran-sepharose: an affinity column for the dinucleotide fold in proteins.
Proc Natl Acad Sci U S A. 1975 Feb;72(2):669-72 PMID: 164664

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.56.6.853

Published

1975-12-00

Pages

853-5

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC541940

External Links

PubMed source DOI: 10.1104/pp.56.6.853 PMC Full Text View journal details

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