Light modulation of enzyme activity: activation of the light effect mediators by reduction and modulation of enzyme activity by thiol-disulfide exchange.

Plant physiology ·Vol. 61 ·No. 4 ·1978-04-00 ·Pages 601-5

Anderson LE, Nehrlich SC, Champigny ML

Abstract

Light and dark modulation experiments with pea (Pisum sativum L.) chloroplast stromal fractions pretreated with dithiothreitol (to reduce protein disulfide bonds) or with 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) (to block sulfhydryl groups) suggest that light modulation involves thiol-disulfide exchange on the modulatable stromal enzyme protein. Light-dependent reduction of DTNB involves a photosynthetic electron transport chain component located on the reducing side of photosystem I prior to ferredoxin; DTNB may be acting as a light effect mediator substitute. The thylakoid-bound light effect mediator system, then, in its light-activated reduced form probably catalyzes thiol-disulfide exchange reactions on stromal enzymes.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Anderson L E

Department of Biological Sciences, University of Illinois at Chicago Circle, Chicago, Illinois 60680.

Nehrlich S C

Champigny M L

References (13)

13 references, click to expand

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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.61.4.601

Published

1978-04-00

Pages

601-5

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1091926

External Links

PubMed source DOI: 10.1104/pp.61.4.601 PMC Full Text View journal details

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Light modulation of enzyme activity: activation of the light effect mediators by reduction and modulation of enzyme activity by thiol-disulfide exchange.

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