Detection of glycosylated and deglycosylated extensin precursors by indirect competitive ELISA.

Plant physiology ·Vol. 83 ·No. 1 ·1987-01-00 ·Pages 1-3

Conrad TA, Lamport DT, Hammerschmidt R

Abstract

A competitive indirect enzyme-linked immunosorbent assay (ELISA) was developed for the rapid quantitation of the glycosylated and deglycosylated forms of the monomeric soluble extensin precursor subunits P1 and P2. A log-linear response range for each kind of precursor in the competition curve was between 0.01 and 100 nanograms per milliliter.

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Conrad T A

Department of Botany and Plant Pathology, Michigan State University, East Lansing, Michigan 48824.

Lamport D T

Hammerschmidt R

References (5)

5 references, click to expand

Purification and Characterization of a Salt-extractable Hydroxyproline-rich Glycoprotein from Aerated Carrot Discs.
Plant Physiol. 1980 Nov;66(5):787-92 PMID: 16661526
Determination of the optimal ammonium sulfate concentration for the fractionation of rabbit, sheep, horse, and goat antisera.
Appl Microbiol. 1973 Jan;25(1):26-36 PMID: 4119831
Hydroxyproline-rich bacterial agglutinin from potato : extraction, purification, and characterization.
Plant Physiol. 1982 Nov;70(5):1353-8 PMID: 16662679
Hydroxyproline arabinosides in the plant kingdom.
Plant Physiol. 1971 Oct;48(4):454-6 PMID: 16657818
A method for producing specific antisera with small doses of immunogen.
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Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.83.1.1

Published

1987-01-00

Pages

1-3

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1056287

External Links

PubMed source DOI: 10.1104/pp.83.1.1 PMC Full Text View journal details

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Detection of glycosylated and deglycosylated extensin precursors by indirect competitive ELISA.

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