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PMID: 16665257 Published · ppublish English Journal Article

Subcellular Metabolite Levels in Spinach Leaves : Regulation of Sucrose Synthesis during Diurnal Alterations in Photosynthetic Partitioning.

Plant physiology ·Vol. 83 ·No. 2 ·1987-02-00 ·Pages 399-407

Gerhardt R, Stitt M, Heldt HW

Abstract

The alterations of subcellular metabolite levels during the day in spinach leaves have been investigated using nonaqueous density gradient centrifugation to separate chloroplasts, cytosol, and vacuole. The results provide direct evidence for the role of sucrose phosphate synthase and cytosolic fructose 1,6-bisphosphatase in regulating sucrose synthesis in leaves and also show that the phosphate translocator is kinetically limiting in vivo.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Gerhardt R
Institut fur Biochemie der Pflanze, Untere Karspule 2, 3400 Gottingen, Federal Republic of Germany.
Stitt M
Heldt H W
References (19)
19 references, click to expand
  1. The distribution of metabolites between spinach chloroplasts and medium during photosynthesis in vitro.
    Biochim Biophys Acta. 1977 May 11;460(2):259-72 PMID: 870037
  2. Regulation of fructose 2,6-bisphosphate concentration in spinach leaves.
    Eur J Biochem. 1984 Aug 15;143(1):89-93 PMID: 6088231
  3. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : III. Properties of the Cytosolic Fructose 1,6-Bisphosphatase.
    Plant Physiol. 1984 Jul;75(3):561-5 PMID: 16663666
  4. Regulation of Sucrose Synthesis by Cytoplasmic Fructosebisphosphatase and Sucrose Phosphate Synthase during Photosynthesis in Varying Light and Carbon Dioxide.
    Plant Physiol. 1983 Jul;72(3):767-74 PMID: 16663083
  5. Intracellular metabolite gradients and flow of carbon during photosynthesis of leaf protoplasts.
    Arch Biochem Biophys. 1980 Nov;205(1):246-59 PMID: 7447481
  6. A role for fructose 2,6-bisphosphate in the regulation of sucrose synthesis in spinach leaves.
    Plant Physiol. 1983 Aug;72(4):1139-41 PMID: 16663136
  7. Localization of Carboxydismutase & Triosephosphate Dehydrogenases in Chloroplasts.
    Plant Physiol. 1963 May;38(3):355-60 PMID: 16655796
  8. Evidence for control of carbon partitioning by fructose 2,6-bisphosphate in spinach leaves.
    Plant Physiol. 1984 Feb;74(2):445-7 PMID: 16663440
  9. Characterization of diurnal changes in activities of enzymes involved in sucrose biosynthesis.
    Plant Physiol. 1983 Oct;73(2):428-33 PMID: 16663233
  10. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : I. Coordination of CO(2) Fixation and Sucrose Synthesis.
    Plant Physiol. 1984 Jul;75(3):548-53 PMID: 16663664
  11. Changes in Starch Formation and Activities of Sucrose Phosphate Synthase and Cytoplasmic Fructose-1,6-bisphosphatase in Response to Source-Sink Alterations.
    Plant Physiol. 1983 Jun;72(2):474-80 PMID: 16663027
  12. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : II. Partitioning between Sucrose and Starch.
    Plant Physiol. 1984 Jul;75(3):554-60 PMID: 16663665
  13. Determination of compartmented metabolite pools by a combination of rapid fractionation of oat mesophyll protoplasts and enzymic cycling.
    Plant Physiol. 1984 Aug;75(4):1017-21 PMID: 16663726
  14. Measurement of subcellular metabolite levels in leaves by fractionation of freeze-stopped material in nonaqueous media.
    Plant Physiol. 1984 Jul;75(3):542-7 PMID: 16663663
  15. Observations on the phosphate status and intracellular pH of intact cells, protoplasts and chloroplasts from photosynthetic tissue using phosphorus-31 nuclear magnetic resonance.
    Biochem J. 1982 Feb 15;202(2):429-34 PMID: 7092824
  16. Alkalization of the chloroplast stroma caused by light-dependent proton flux into the thylakoid space.
    Biochim Biophys Acta. 1973 Aug 31;314(2):224-41 PMID: 4747067
  17. Control of Photosynthetic Sucrose Synthesis by Fructose 2,6-Bisphosphate : V. Modulation of the Spinach Leaf Cytosolic Fructose 1,6-Bisphosphatase Activity in Vitro by Substrate, Products, pH, Magnesium, Fructose 2,6-Bisphosphate, Adenosine Monophosphate, and Dihydroxyacetone Phosphate.
    Plant Physiol. 1985 Nov;79(3):590-8 PMID: 16664457
  18. Metabolite levels during induction in the chloroplast and extrachloroplast compartments of spinach protoplasts.
    Biochim Biophys Acta. 1980 Nov 5;593(1):85-102 PMID: 7426648
  19. Changes of Sucrose-Phosphate Synthase Activity in Barley Primary Leaves during Light/Dark Transitions.
    Plant Physiol. 1984 Dec;76(4):910-2 PMID: 16663969
Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
Published
1987-02-00
Pages
399-407
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1056369
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