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PMID: 16665544 Published · ppublish English Journal Article

Temperature-induced protein conformational changes in barley root plasma membrane-enriched microsomes: I. Effect of temperature on membrane protein and lipid mobility.

Plant physiology ·Vol. 84 ·No. 3 ·1987-07-00 ·Pages 918-23

Caldwell CR, Whitman CE

Abstract

A protein spin label and lipid spin probes were used to study the temperature-dependent motion of protein and lipid, respectively, in barley (Hordeum vulgare L. cv Conquest) root plasma membrane-enriched microsomes. Using membranes from seedlings grown at 20 degrees C, the temperature-dependence of the relative motion of membrane surface spin probes and a spin label covalently attached to membrane proteins suggested abrupt changes in the lipid and protein mobilities at about 12 degrees C. Spin probe spin-spin exchange broadening and fluorescent probe eximer formation indicated apparent temperature-induced alterations in probe lateral diffusion within the membrane at about 12 to 14 degrees C. The results suggest the presence of temperature-induced quasicrystalline lipid clusters which may influence the activity of membrane-bound enzymes.

Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Caldwell C R
Plant Stress Laboratory, United States Department of Agriculture, Beltsville, Maryland 20705.
Whitman C E
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Article Info
Journal
Plant physiology
Abbr.
Plant Physiol
ISSN
0032-0889
DOI
10.1104/pp.84.3.918
Published
1987-07-00
Pages
918-23
Language
English
Region
United States
NLM ID
0401224
PMCID
PMC1056695
External Links
PubMed source DOI: 10.1104/pp.84.3.918 PMC Full Text View journal details
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