A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis.

Plant physiology ·Vol. 86 ·No. 3 ·1988-03-00 ·Pages 822-5

Chen KH, Miller AN, Patterson GW, Cohen JD

Abstract

A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Chen K H

Department of Botany, University of Maryland, College Park, Maryland 20742.

Miller A N

Patterson G W

Cohen J D

References (10)

10 references, click to expand

Isolation of indole-3-acetic acid from corn kernels & etiolated corn seedlings.
Plant Physiol. 1961 May;36(3):354-9 PMID: 16655520
Effect of Deseeding on the Indole-3-acetic Acid Content of Shoots and Roots of Zea mays Seedlings.
Plant Physiol. 1983 Jun;72(2):526-9 PMID: 16663036
Distribution of Free and Ester Indole-3-Acetic Acid in the Cortex and Stele of the Zea mays Mesocotyl.
Plant Physiol. 1982 Jun;69(6):1304-7 PMID: 16662391
Gravitational effects on plant growth hormone concentration.
Adv Space Res. 1983;3(9):229-35 PMID: 11542452
Synthesis of 4,5,6,7 and 2,4,5,6,7 Deuterium-labeled Indole-3-Acetic Acid for Use in Mass Spectrometric Assays.
Plant Physiol. 1980 Oct;66(4):775-81 PMID: 16661520
Double-standard isotope dilution assay. I. Quantitative assay of indole-3-acetic acid.
Anal Biochem. 1981 Apr;112(2):249-57 PMID: 7020481
Endogenous indole-3-acetic Acid in the stem of tobacco in relation to flower neoformation as measured by mass spectroscopic assay.
Plant Physiol. 1984 May;75(1):257-60 PMID: 16663588
Measurement of Indole-3-Acetic Acid in Peach Fruits (Prunus persica L. Batsch cv Redhaven) during Development.
Plant Physiol. 1987 Jun;84(2):491-4 PMID: 16665467
C(6)-[benzene ring]-indole-3-acetic Acid: a new internal standard for quantitative mass spectral analysis of indole-3-acetic Acid in plants.
Plant Physiol. 1986 Jan;80(1):14-9 PMID: 16664570
Investigations on the Nature of the Auxin-Wave in the Cambial Region of Pine Stems : Validation of IAA as the Auxin Component by the Avena Coleoptile Curvature Assay and by Gas Chromatography-Mass Spectrometry-Selected Ion Monitoring.
Plant Physiol. 1987 May;84(1):135-43 PMID: 16665387

Article Info

Journal

Plant physiology

Abbr.

Plant Physiol

ISSN

0032-0889

DOI

10.1104/pp.86.3.822

Published

1988-03-00

Pages

822-5

Language

English

Region

United States

NLM ID

0401224

PMCID

PMC1054577

External Links

PubMed source DOI: 10.1104/pp.86.3.822 PMC Full Text View journal details

Analysis Services

A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis.

Contact

WeChat Official Account