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PMID: 16688221 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A dynein loading zone for retrograde endosome motility at microtubule plus-ends.

The EMBO journal ·Vol. 25 ·No. 11 ·2006-06-07 ·Pages 2275-86

Lenz JH, Schuchardt I, Straube A, Steinberg G

Abstract

In the fungus Ustilago maydis, early endosomes move bidirectionally along microtubules (MTs) and facilitate growth by local membrane recycling at the tip of the infectious hypha. Here, we set out to elucidate the molecular mechanism of this process. We show that endosomes travel by Kinesin-3 activity into the hyphal apex, where they reverse direction and move backwards in a dynein-dependent manner. Our data demonstrate that dynein, dynactin and Lis1 accumulate at MT plus-ends within the hyphal tip, where they provide a reservoir of inactive motors for retrograde endosome transport. Consistently, endosome traffic is abolished after depletion of the dynein activator Lis1 and in Kinesin-1 null mutants, which was due to a defect in targeting of dynein and dynactin to the apical MT plus-ends. Furthermore, biologically active GFP-dynein travels on endosomes in retrograde and not in anterograde direction. Surprisingly, a CLIP170 homologue was neither needed for dynein localization nor for endosome transport. These results suggest an apical dynein loading zone in the hyphal tip, which ensure that endosomes reach the expanding growth region before they reverse direction.

MeSH Terms
Biological Transport/physiology Dynactin Complex Dyneins/genetics,metabolism Endosomes/metabolism Fungal Proteins/metabolism Hyphae/metabolism,ultrastructure Kinesins/genetics,metabolism Microtubule-Associated Proteins/genetics,metabolism Microtubules/metabolism,ultrastructure Neoplasm Proteins/genetics,metabolism Protein Isoforms/genetics,metabolism Recombinant Fusion Proteins/genetics,metabolism Ustilago/cytology,genetics,metabolism
Chemicals
Dynactin Complex Fungal Proteins Microtubule-Associated Proteins Neoplasm Proteins Protein Isoforms Recombinant Fusion Proteins cytoplasmic linker protein 170 Dyneins Kinesins
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Lenz J H
Max-Planck-Institut für terrestrische Mikrobiologie, Marburg, Germany.
Schuchardt I
Straube A
Steinberg G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
DOI
10.1038/sj.emboj.7601119
Published
2006-06-07
Epub
2006-00-11
Pages
2275-86
Language
English
Region
England
NLM ID
8208664
PMCID
PMC1478194
Subset
IM
External Links
PubMed source DOI: 10.1038/sj.emboj.7601119 PMC Full Text View journal details
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