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PMID: 1671715 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 4 ·1991-02-15 ·Pages 1384-8

Singh MB, Hough T, Theerakulpisut P, Avjioglu A, Davies S, Smith PM, Taylor P, Simpson RJ, Ward LD, McCluskey J

Abstract

We have identified a major allergenic protein from rye-grass pollen, tentatively designated Lol pIb of 31kDa and with pI 9.0. A cDNA clone encoding Lol pIb has been isolated, sequenced, and characterized. Lol pIb is located mainly in the starch granules. This is a distinct allergen from Lol pI, which is located in the cytosol. Lol pIb is synthesized in pollen as a pre-allergen with a transit peptide targeting the allergen to amyloplasts. Epitope mapping of the fusion protein localized the IgE binding determinant in the C-terminal domain.

MeSH Terms

Allergens Amino Acid Sequence Antibodies, Monoclonal Antigens, Plant Base Sequence Cloning, Molecular DNA/genetics,isolation & purification Gene Library Lolium/genetics,immunology Molecular Sequence Data Plant Proteins/genetics,immunology Pollen/analysis,immunology,ultrastructure Poly A/genetics,isolation & purification Protein Conformation RNA, Messenger/genetics,isolation & purification Restriction Mapping

Chemicals

Allergens Antibodies, Monoclonal Antigens, Plant Lol p I protein, Lolium perenne Plant Proteins RNA, Messenger Poly A DNA

Authors & Affiliations

10 authors, click to expand affiliations / ORCID

Singh M B

School of Botany, University of Melbourne, Parkville, Victoria, Australia.

Hough T

Theerakulpisut P

Avjioglu A

Davies S

Smith P M

Taylor P

Simpson R J

Ward L D

McCluskey J

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21 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.88.4.1384

Published

1991-02-15

Pages

1384-8

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC51022

Subset

Databases

GENBANK

M59163

External Links

PubMed source DOI: 10.1073/pnas.88.4.1384 PMC Full Text View journal details

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Isolation of cDNA encoding a newly identified major allergenic protein of rye-grass pollen: intracellular targeting to the amyloplast.

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