The polyphosphoinositide content of the leucocyte, erythrocyte and macrophage.

The Biochemical journal ·Vol. 105 ·No. 3 ·1967-12-00 ·Pages 1039-45

Wieneke AA, Woodin AM

Abstract

1. The polyphosphoinositide content of macrophages and the cell membranes of leucocytes and erythrocytes was determined by an extension of the ;acid-hydrolysis' procedure of Dawson & Eichberg (1965). The estimation was controlled by adding a little highly radioactive polyphosphoinositide to the tissue extracts before fractionation. Several standard methods for determining polyphosphoinositides gave low recoveries when applied to leucocytes, and it is suggested that these cells contain materials that form complexes with the polyphosphoinositides and interfere with the assay. 2. The method for the preparation of leucocyte cell surface membranes has been modified.

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Wieneke A A

Sir William Dunn School of Pathology, University of Oxford.

Woodin A M

References (13)

13 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1051039

Published

1967-12-00

Pages

1039-45

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1198423

External Links

PubMed source DOI: 10.1042/bj1051039 PMC Full Text View journal details

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The polyphosphoinositide content of the leucocyte, erythrocyte and macrophage.

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