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PMID: 1674369 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A transposon-related palindromic repetitive sequence from C. elegans.

Nucleic acids research ·Vol. 19 ·No. 8 ·1991-04-25 ·Pages 1871-7

Dreyfus DH, Emmons SW

Abstract

A family of transposon-like sequences in the C. elegans genome is described. This family, termed the Tc6 family, consists mostly of conserved, 1.6 kb elements. Four Tc6 elements or partial elements have been cloned and the DNA sequences of three were determined. One appears to be a complete element of 1603 nucleotides, consisting of a palindrome of 765 nucleotides, with a central, non-palindromic region of 73 nucleotides. Another has an identical structure except for an internal deletion. A third is a partial element terminating at a probable internal restriction site used for cloning. A fourth clone contained portions of the Tc6 sequence juxtaposed to non-Tc6 sequences. All C. elegans strains examined contain 20-30 Tc6 elements. The ends of Tc6 elements are conserved and have sequence similarity to the ends of C. elegans transposons Tc1 and Tc3. The ends of Tc6 elements also have sequence similarity to the heptamer portion of the immunoglobulin and T-cell receptor recombination signal sequence, raising the possibility of wide phylogenetic conservation of the recombination mechanism. Tc6 elements also share sequence motifs with plant-pathogenic viroid RNA's, possibly indicative of a Tc6 RNA replicative phase.

MeSH Terms
Animals Base Sequence Blotting, Southern Caenorhabditis/genetics DNA DNA Transposable Elements Molecular Sequence Data Polymorphism, Restriction Fragment Length Repetitive Sequences, Nucleic Acid Sequence Alignment
Chemicals
DNA Transposable Elements DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Dreyfus D H
Department of Molecular Genetics, Albert Einstein College of Medicine, Bronx, NY 10461.
Emmons S W
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/19.8.1871
Published
1991-04-25
Pages
1871-7
Language
English
Region
England
NLM ID
0411011
PMCID
PMC328117
Subset
IM
Grants
NIGMS NIH HHS · GM38174 · United States
NIGMS NIH HHS · T32 GM07288 · United States
Databases
GENBANK
X15927, X15928, X15929, X15930, X15931, X15932, X15933, X15934, X15935, X55356
External Links
PubMed source DOI: 10.1093/nar/19.8.1871 PMC Full Text View journal details
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