Preparation and functional assay of pure populations of tyrosinated and detyrosinated tubulin.

Alkaloids/pharmacology Animals Brain/metabolism,ultrastructure Cattle Cell Fractionation/methods Chromatography, Ion Exchange Electrophoresis, Polyacrylamide Gel HeLa Cells/metabolism Humans Indicators and Reagents Macromolecular Substances Microtubule-Associated Proteins/isolation & purification Microtubules/drug effects,ultrastructure Molecular Weight Neurons/metabolism,ultrastructure Paclitaxel Tubulin/isolation & purification,metabolism Tyrosine Ultracentrifugation/methods

Alkaloids Indicators and Reagents Macromolecular Substances Microtubule-Associated Proteins Tubulin Tyrosine Paclitaxel