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PMID: 16749169 Published · ppublish English Journal Article

Steady-state concentrations of coenzyme A, acetyl-coenzyme A and long-chain fatty acyl-coenzyme A in rat-liver mitochondria oxidizing palmitate.

The Biochemical journal ·Vol. 97 ·No. 2 ·1965-11-00 ·Pages 587-94

Garland PB, Shepherd D, Yates DW

Abstract

1. Fluorimetric assays are described for CoASH, acetyl-CoA and long-chain fatty acyl-CoA, and are sensitive to at least 50mumumoles of each. 2. Application of these assays to rat-liver mitochondria oxidizing palmitate in the absence and presence of carnitine indicated two pools of intramitochondrial CoA. One pool could be acylated by palmitate and ATP, and the other pool acylated by palmitate with ATP and carnitine, or by palmitoylcarnitine alone. 3. The intramitochondrial content of acetyl-CoA is increased by the oxidation of palmitate both in the absence and presence of l-malate. 4. The conversion of palmitoyl-CoA into acetyl-CoA by beta-oxidation takes place without detectable accumulation of acyl-CoA intermediates.

Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Garland P B
Department of Biochemistry, University of Bristol.
Shepherd D
Yates D W
References (21)
21 references, click to expand
  1. Studies on alpha-ketoglutaric oxidase. II. Purification and properties.
    J Biol Chem. 1952 May;197(2):851-62 PMID: 12981117
  2. Fatty acid oxidation by a soluble enzyme system from mitochondria.
    J Biol Chem. 1953 May;202(1):119-36 PMID: 13061438
  3. Functional group of coenzyme A and its metabolic relations, especially in the fatty acid cycle.
    Fed Proc. 1953 Sep;12(3):683-91 PMID: 13107740
  4. Studies on the fatty acid oxidizing system of animal tissues. VII. The beta-ketoacyl coenzyme A cleavage enzyme.
    J Biol Chem. 1954 May;208(1):345-57 PMID: 13174544
  5. Tissue sulfhydryl groups.
    Arch Biochem Biophys. 1959 May;82(1):70-7 PMID: 13650640
  6. Evidence against free butyryl-CoA as an intermediate in long chain fatty acid biosynthesis.
    Biochem Biophys Res Commun. 1961 May 15;5:16-21 PMID: 13767324
  7. Studies on the mechanism of fatty acid synthesis. XI. The product of the reaction and the role of sulfhydryl groups in the synthesis of fatty acids.
    J Biol Chem. 1962 May;237:1441-8 PMID: 13872874
  8. A fluorimetric method for the quantitative microanalysis of adenine and pyridine nucleotides.
    Anal Biochem. 1962 May;3:369-82 PMID: 13890835
  9. Specificity of carnitine action on fatty acid oxidation by heart muscle.
    Am J Physiol. 1962 Jan;202:117-21 PMID: 13895427
  10. The condensation reaction of fatty acid synthesis. I. Separation and properties of the enzymes.
    J Biol Chem. 1963 Feb;238:557-65 PMID: 14011744
  11. Carnitine in intermediary metabolism. The metabolism of fatty acid esters of carnitine by mitochondria.
    J Biol Chem. 1962 Dec;237:3628-32 PMID: 14015219
  12. The formation of malonyl-enzyme and its conversion to fatty acids and beta-hydroxy, beta-methyl glutaryl coenzyme A.
    Biochem Biophys Res Commun. 1963 Jul 10;12:27-33 PMID: 14015676
  13. INHIBITION OF CITRATE-SYNTHASE BY PALMITYL-COENZYME A.
    Biochem Biophys Res Commun. 1963 Sep 10;13:26-31 PMID: 14069507
  14. INHIBITION OF CITRATE FORMATION BY LONG-CHAIN ACYL THIOESTERS OF COENZYME A AS A POSSIBLE CONTROL MECHANISM IN FATTY ACID BIOSYNTHESIS.
    Biochim Biophys Acta. 1963 Oct 22;70:608-9 PMID: 14085951
  15. LONG-CHAIN CARNITINE ACYLTRANSFERASE AND THE ROLE OF ACYLCARNITINE DERIVATIVES IN THE CATALYTIC INCREASE OF FATTY ACID OXIDATION INDUCED BY CARNITINE.
    J Lipid Res. 1963 Jul;4:279-88 PMID: 14168165
  16. EFFECT OF CARNITINE ON THE OXIDATION OF ALPHA-OXOGLUTARATE TO SUCCINATE IN THE PRESENCE OF ACETOACETATE OR PYRUVATE.
    Biochim Biophys Acta. 1964 Oct 9;93:166-8 PMID: 14249143
  17. THE EFFECT OF ATRACTYLATE AND OLIGOMYCIN ON THE BEHAVIOUR OF MITOCHONDRIA TOWARDS ADENINE NUCLEOTIDES.
    Biochem J. 1965 Jun;95:707-16 PMID: 14342506
  18. The composition of the ketoglutarate dehydrogenase complex.
    Biochim Biophys Acta. 1960 Mar 11;38:447-60 PMID: 14422131
  19. Biosynthesis of saturated fatty acids.
    Fed Proc. 1961 Dec;20:941-51 PMID: 14467591
  20. The oxidation of citrate, isocitrate and cis-aconitate by isolated mitochondria.
    Biochem J. 1964 Feb;90(2):225-37 PMID: 4378636
  21. Variations in tissue contents of coenzyme A thio esters and possible metabolic implications.
    Biochem J. 1964 Dec;93(3):550-7 PMID: 5839190
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj0970587
Published
1965-11-00
Pages
587-94
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1264680
External Links
PubMed source DOI: 10.1042/bj0970587 PMC Full Text View journal details
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