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PMID: 16769775 Published · epublish English Journal Article Research Support, Non-U.S. Gov't Review

Xp54 and related (DDX6-like) RNA helicases: roles in messenger RNP assembly, translation regulation and RNA degradation.

Nucleic acids research ·Vol. 34 ·No. 10 ·2006-00-00 ·Pages 3082-94

Weston A, Sommerville J

Abstract

The DEAD-box RNA helicase Xp54 is an integral component of the messenger ribonucleoprotein (mRNP) particles of Xenopus oocytes. In oocytes, several abundant proteins bind pre-mRNA transcripts to modulate nuclear export, RNA stability and translational fate. Of these, Xp54, the mRNA-masking protein FRGY2 and its activating protein kinase CK2alpha, bind to nascent transcripts on chromosome loops, whereas an Xp54-associated factor, RapA/B, binds to the mRNP complex in the cytoplasm. Over-expression, mutation and knockdown experiments indicate that Xp54 functions to change the conformation of mRNP complexes, displacing one subset of proteins to accommodate another. The sequence of Xp54 is highly conserved in a wide spectrum of organisms. Like Xp54, Drosophila Me31B and Caenorhabditis CGH-1 are required for proper meiotic development, apparently by regulating the translational activation of stored mRNPs and also for sorting certain mRNPs into germplasm-containing structures. Studies on yeast Dhh1 and mammalian rck/p54 have revealed a key role for these helicases in mRNA degradation and in earlier remodelling of mRNP for entry into translation, storage or decay pathways. The versatility of Xp54 and related helicases in modulating the metabolism of mRNAs at all stages of their lifetimes marks them out as key regulators of post-transcriptional gene expression.

MeSH Terms
Active Transport, Cell Nucleus Amino Acid Sequence Animals Cell Cycle Cell Nucleus/metabolism DEAD-box RNA Helicases Gene Expression Regulation Humans Molecular Sequence Data Protein Biosynthesis Proto-Oncogene Proteins/metabolism RNA Helicases/chemistry,physiology RNA Nucleotidyltransferases/metabolism RNA Stability RNA, Messenger/metabolism Ribonucleoproteins/metabolism Xenopus Proteins/chemistry,physiology
Chemicals
Proto-Oncogene Proteins RNA, Messenger Ribonucleoproteins Xenopus Proteins messenger ribonucleoprotein RNA Nucleotidyltransferases DDX6 protein, human Xp54 RNA helicase, Xenopus DEAD-box RNA Helicases RNA Helicases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Weston Andrew
School of Biology, University of St Andrews, St Andrews, Fife KY16 9TS, Scotland, UK.
Sommerville John
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkl409
Published
2006-00-00
Epub
2006-00-12
Pages
3082-94
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1477856
Subset
IM
Grants
Wellcome Trust · United Kingdom
External Links
PubMed source DOI: 10.1093/nar/gkl409 PMC Full Text View journal details
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