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PMID: 16769819 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcription of ribosomal genes can cause nondisjunction.

The Journal of cell biology ·Vol. 173 ·No. 6 ·2006-06-19 ·Pages 893-903

Machín F, Torres-Rosell J, De Piccoli G, Carballo JA, Cha RS, Jarmuz A, Aragón L

Abstract

Mitotic disjunction of the repetitive ribosomal DNA (rDNA) involves specialized segregation mechanisms dependent on the conserved phosphatase Cdc14. The reason behind this requirement is unknown. We show that rDNA segregation requires Cdc14 partly because of its physical length but most importantly because a fraction of ribosomal RNA (rRNA) genes are transcribed at very high rates. We show that cells cannot segregate rDNA without Cdc14 unless they undergo genetic rearrangements that reduce rDNA copy number. We then demonstrate that cells with normal length rDNA arrays can segregate rDNA in the absence of Cdc14 as long as rRNA genes are not transcribed. In addition, our study uncovers an unexpected role for the replication barrier protein Fob1 in rDNA segregation that is independent of Cdc14. These findings demonstrate that highly transcribed loci can cause chromosome nondisjunction.

MeSH Terms
Cell Cycle Proteins/genetics,physiology Chromosome Segregation DNA, Ribosomal/genetics Fungal Proteins/genetics,physiology Gene Conversion/physiology Gene Deletion Gene Dosage Genes, cdc Genes, rRNA Models, Genetic Mutation Nondisjunction, Genetic RNA Polymerase II/metabolism RNA, Ribosomal/biosynthesis Transcription, Genetic/physiology Yeasts/cytology
Chemicals
Cell Cycle Proteins DNA, Ribosomal Fungal Proteins RNA, Ribosomal RNA Polymerase II
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Machín Felix
Cell Cycle Group, Medical Research Council Clinical Sciences Centre, Imperial College London, London W12 0NN, England, UK.
Torres-Rosell Jordi
De Piccoli Giacomo
Carballo Jesús A
Cha Rita S
Jarmuz Adam
Aragón Luis
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.200511129
Published
2006-06-19
Epub
2006-00-12
Pages
893-903
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2063915
Subset
IM
Grants
Medical Research Council · MC_U117573806 · United Kingdom
Medical Research Council · MC_U120074328 · United Kingdom
External Links
PubMed source DOI: 10.1083/jcb.200511129 PMC Full Text View journal details
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