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PMID: 16775685 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Identification of quantitative trait loci and associated candidate genes for low-temperature tolerance in cold-hardy winter wheat.

Functional & integrative genomics ·Vol. 7 ·No. 1 ·2007-01-00 ·Pages 53-68

Båga M, Chodaparambil SV, Limin AE, Pecar M, Fowler DB, Chibbar RN

Abstract

Low-temperature (LT) tolerance is an important economic trait in winter wheat (Triticum aestivum L.) that determines the plants' ability to cope with below freezing temperatures. Essential elements of the LT tolerance mechanism are associated with the winter growth habit controlled by the vernalization loci (Vrn-1) on the group 5 chromosomes. To identify genomic regions, which in addition to vrn-1 determine the level of LT tolerance in hexaploid wheat, two doubled haploid (DH) mapping populations were produced using parents with winter growth habit (vrn-A1, vrn-B1, and vrn-D1) but showing different LT tolerance levels. A total of 107 DH lines were analyzed by genetic mapping to produce a consensus map of 2,873 cM. The LT tolerance levels for the Norstar (LT(50)=-20.7 degrees C) x Winter Manitou (LT(50)=-14.3 degrees C) mapping population ranged from -12.0 to -22.0 degrees C. Single marker analysis and interval mapping of phenotyped lines revealed a major quantitative trait locus (QTL) on chromosome 5A and a weaker QTL on chromosome 1D. The 5A QTL located 46 cM proximal to the vrn-A1 locus explained 40% of the LT tolerance variance. Two C-repeat Binding Factor (CBF) genes expressed during cold acclimation in Norstar were located at the peak of the 5A QTL.

MeSH Terms
Cold Temperature Genetic Markers MADS Domain Proteins/genetics Plant Proteins/chemistry,genetics,physiology Quantitative Trait Loci/genetics Triticum/chemistry,genetics,physiology
Chemicals
Genetic Markers MADS Domain Proteins Plant Proteins
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Båga Monica
Department of Plant Sciences, University of Saskatchewan, 51 Campus Drive, Saskatoon, Saskatchewan, S7N 5A8, Canada.
Chodaparambil Sanjay V
Limin Allen E
Pecar Marin
Fowler D Brian
Chibbar Ravindra N
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Article Info
Journal
Functional & integrative genomics
Abbr.
Funct Integr Genomics
ISSN
1438-793X
DOI
10.1007/s10142-006-0030-7
Published
2007-01-00
Epub
2006-00-15
Pages
53-68
Language
English
Region
Germany
NLM ID
100939343
Subset
IM
External Links
PubMed source DOI: 10.1007/s10142-006-0030-7 View journal details
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