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PMID: 16782812 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

A humanized gnotobiotic mouse model of host-archaeal-bacterial mutualism.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 26 ·2006-06-27 ·Pages 10011-6

Samuel BS, Gordon JI

Abstract

Our colons harbor trillions of microbes including a prominent archaeon, Methanobrevibacter smithii. To examine the contributions of Archaea to digestive health, we colonized germ-free mice with Bacteroides thetaiotaomicron, an adaptive bacterial forager of the polysaccharides that we consume, with or without M. smithii or the sulfate-reducing bacterium Desulfovibrio piger. Whole-genome transcriptional profiling of B. thetaiotaomicron, combined with mass spectrometry, revealed that, unlike D. piger, M. smithii directs B. thetaiotaomicron to focus on fermentation of dietary fructans to acetate, whereas B. thetaiotaomicron-derived formate is used by M. smithii for methanogenesis. B. thetaiotaomicron-M. smithii cocolonization produces a significant increase in host adiposity compared with monoassociated, or B. thetaiotaomicron-D. piger biassociated, animals. These findings demonstrate a link between this archaeon, prioritized bacterial utilization of polysaccharides commonly encountered in our modern diets, and host energy balance.

MeSH Terms
Animals Bacteroides/genetics,metabolism Colon/microbiology,physiology Desulfovibrio/metabolism,physiology Diet Digestion/physiology Fermentation Fructans/metabolism Germ-Free Life Humans Methanobrevibacter/physiology Mice Molecular Sequence Data Polysaccharides/metabolism Symbiosis
Chemicals
Fructans Polysaccharides levan
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Samuel Buck S
Center for Genome Sciences, Washington University School of Medicine, 4444 Forest Park, St. Louis, MO 63108, USA.
Gordon Jeffrey I
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0602187103
Published
2006-06-27
Epub
2006-00-16
Pages
10011-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1479766
Subset
IM
Grants
NIDDK NIH HHS · R01 DK030292 · United States
NIDDK NIH HHS · R01 DK070977 · United States
NIDDK NIH HHS · DK 30292 · United States
NIDDK NIH HHS · DK 70977 · United States
NIDDK NIH HHS · R37 DK030292 · United States
Databases
GENBANK
DQ419923
External Links
PubMed source DOI: 10.1073/pnas.0602187103 PMC Full Text View journal details
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