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PMID: 168110 Published · ppublish English Journal Article

A radioreceptor-assay for glucagon: binding of enteroglucagon to liver plasma membranes.

Diabetologia ·Vol. 11 ·No. 3 ·1975-06-00 ·Pages 211-9

Holst JJ

Abstract

A radioreceptor-assay for glucagon was developed employing pig liver plasma membranes isolated by means of an aqueous two-phase polymer system. The assay is simple, precise, and has a detection limit of 40 pg/ml. Acid-ethanol extracts of porcine intestinal mucosa and entero-glucagon purified by affinity chromatography interfered strongly with the binding of 125I-labelled glucagon. The affinity of enteroglucagon for the membranes was lower than that of glucagon, but even physiological concentrations of the former interfere with glucagon binding, indicating that enteroglucagon may compete with pancreatic glucagon for binding to the hepatocyte under physiological conditions.

MeSH Terms
Adsorption Animals Binding, Competitive Cell Membrane/metabolism Chromatography, Affinity Gastrointestinal Hormones/isolation & purification,metabolism Glucagon/analysis Immune Sera Liver/metabolism Radioimmunoassay Radioligand Assay/methods Receptors, Cell Surface Swine
Chemicals
Gastrointestinal Hormones Immune Sera Receptors, Cell Surface Glucagon
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Holst J J
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36 references, click to expand
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Article Info
Journal
Diabetologia
Abbr.
Diabetologia
ISSN
0012-186X
DOI
10.1007/BF00422324
Published
1975-06-00
Pages
211-9
Language
English
Region
Germany
NLM ID
0006777
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00422324 View journal details
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