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PMID: 1681549 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Wegener autoantigen and myeloblastin are encoded by a single mRNA.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 88 ·No. 20 ·1991-10-15 ·Pages 9253-6

Labbaye C, Musette P, Cayre YE

Abstract

Myeloblastin is a serine protease that has been identified in the human leukemia cell line HL-60. Down-regulation of this protease can inhibit proliferation and induce differentiation of promyelocyte-like human leukemic cells. Proteinase 3, a serine protease of human neutrophils, has been identified as the Wegener autoantigen. A high level of homology between myeloblastin and proteinase 3 has suggested that they may be a single serine protease. We have recently completed the 5'-terminal nucleotide sequence of proteinase 3 and shown that its mRNA was also expressed in HL-60 cells and in cells from patients with acute myeloid leukemia. Here we demonstrate that myeloblastin and proteinase 3 are encoded by a single mRNA.

MeSH Terms
Autoantigens/genetics Base Sequence Blotting, Northern Blotting, Southern Cell Line DNA Probes DNA, Neoplasm/genetics,isolation & purification Humans Leukemia Molecular Sequence Data Myeloblastin Oligodeoxyribonucleotides Oligonucleotides, Antisense Poly A/genetics,isolation & purification Polymerase Chain Reaction RNA, Messenger/genetics,isolation & purification Restriction Mapping Serine Endopeptidases/genetics
Chemicals
Autoantigens DNA Probes DNA, Neoplasm Oligodeoxyribonucleotides Oligonucleotides, Antisense RNA, Messenger Poly A Serine Endopeptidases Myeloblastin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Labbaye C
Department of Physiology and Cellular Biophysics, Columbia University College of Physicians and Surgeons, New York, NY 10032.
Musette P
Cayre Y E
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.88.20.9253
Published
1991-10-15
Pages
9253-6
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC52692
Subset
IM
Databases
GENBANK
M75154, S60069, S60071, S60073, S60076, S60079, S60081, S60988, S61137, S61158
External Links
PubMed source DOI: 10.1073/pnas.88.20.9253 PMC Full Text View journal details
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