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PMID: 16818385 Published · ppublish English Journal Article Research Support, N.I.H., Extramural

Dynamic imaging of cerebellar Purkinje cells reveals a population of filopodia which cross-link dendrites during early postnatal development.

Cerebellum (London, England) ·Vol. 5 ·No. 2 ·2006-00-00 ·Pages 105-15

Sdrulla AD, Linden DJ

Abstract

Two-photon microscopy was used to image dye-loaded filopodia of Purkinje cells in acute rat cerebellar slices. In the process of examining filopodia in Purkinje cells from a period of rapid dendritic growth (P10-21), we observed a small subset of filopodia which appeared to form connections between two dendrites of the same cell, usually between the tips of two adjacent dendrites or the tip of a dendrite and the shaft of another. There were fewer of these 'filopodial bridges' present at P18-21 than at an earlier stage in development (P10-12) and they were absent in mature Purkinje cells. Filopodial bridges do not appear to be an artifact of living brain slice preparation as they may also be seen by dye-loading Purkinje cells in slices prepared from perfusion-fixed brain. They have varied morphologies which are mostly similar to conventional, unattached filopodia. However, when measured over tens of minutes, filopodial bridges were observed to be less motile than conventional filopodia as indicated by a reduced index of expansion. While the functions of these novel structures are unknown it is attractive to speculate that they play an instructive role in Purkinje cell dendritic development.

MeSH Terms
Animals Artifacts Cell Differentiation/physiology Cerebellar Cortex/cytology,growth & development Dendrites/physiology,ultrastructure Mice Mice, Inbred ICR Microscopy, Confocal/methods Organ Culture Techniques Organic Chemicals Patch-Clamp Techniques Pseudopodia/physiology,ultrastructure Purkinje Cells/cytology,physiology Rats Rats, Sprague-Dawley Staining and Labeling/methods Tissue Fixation/standards
Chemicals
Alexa594 Organic Chemicals
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Sdrulla Andrei D
Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, Maryland 21205, USA.
Linden David J
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Article Info
Journal
Cerebellum (London, England)
Abbr.
Cerebellum
ISSN
1473-4222
DOI
10.1080/14734220600620908
Published
2006-00-00
Pages
105-15
Language
English
Region
United States
NLM ID
101089443
Subset
IM
Grants
NIMH NIH HHS · R37 MH51106 · United States
External Links
PubMed source DOI: 10.1080/14734220600620908 View journal details
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