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PMID: 16818672 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Interleukin 12p40 is required for dendritic cell migration and T cell priming after Mycobacterium tuberculosis infection.

The Journal of experimental medicine ·Vol. 203 ·No. 7 ·2006-07-10 ·Pages 1805-15

Khader SA, Partida-Sanchez S, Bell G, Jelley-Gibbs DM, Swain S, Pearl JE, Ghilardi N, Desauvage FJ, Lund FE, Cooper AM

Abstract

Migration of dendritic cells (DCs) to the draining lymph node (DLN) is required for the activation of naive T cells. We show here that migration of DCs from the lung to the DLN after Mycobacterium tuberculosis (Mtb) exposure is defective in mice lacking interleukin (IL)-12p40. This defect compromises the ability of IL-12p40-deficient DCs to activate naive T cells in vivo; however, DCs that express IL-12p40 alone can activate naive T cells. Treatment of IL-12p40-deficient DCs with IL-12p40 homodimer (IL-12(p40)(2)) restores Mtb-induced DC migration and the ability of IL-12p40-deficient DCs to activate naive T cells. These data define a novel and fundamental role for IL-12p40 in the pathogen-induced activation of pulmonary DCs.

MeSH Terms
Animals CD4-Positive T-Lymphocytes/immunology Cell Movement/immunology Cells, Cultured Dendritic Cells/cytology,immunology,metabolism Interleukin-12/deficiency,genetics,physiology Interleukin-12 Subunit p40 Lymph Nodes/cytology,immunology Lymphocyte Activation/immunology Mice Mice, Inbred C57BL Mice, Knockout Mycobacterium tuberculosis/immunology Protein Subunits/deficiency,genetics,physiology Tuberculosis, Pulmonary/immunology,microbiology,pathology
Chemicals
Interleukin-12 Subunit p40 Protein Subunits Interleukin-12
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Khader Shabaana A
Trudeau Institute, Inc., Saranac Lake, NY 12983, USA.
Partida-Sanchez Santiago
Bell Guy
Jelley-Gibbs Dawn M
Swain Susan
Pearl John E
Ghilardi Nico
Desauvage Frederic J
Lund Frances E
Cooper Andrea M
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.20052545
Published
2006-07-10
Epub
2006-00-03
Pages
1805-15
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2118335
Subset
IM
Grants
NIAID NIH HHS · P01 AI046530 · United States
NIAID NIH HHS · AI46530 · United States
External Links
PubMed source DOI: 10.1084/jem.20052545 PMC Full Text View journal details
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