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PMID: 1682304 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Characterization of the groEL-like genes in Streptomyces albus.

Journal of bacteriology ·Vol. 173 ·No. 22 ·1991-11-00 ·Pages 7382-6

Mazodier P, Guglielmi G, Davies J, Thompson CJ

Abstract

Three GroEL-like heat shock proteins (HSP56, HSP58, and HSP18) have been observed in Streptomyces albus (G. Guglielmi, P. Mazodier, C. J. Thompson, and J. Davies, J. Bacteriol. 173:7374-7381, 1991). Here we report the cloning and complete nucleotide sequence of groEL1, which encodes HSP18 and HSP58, and groEL2, which encodes HSP56. Both nucleotide sequences predicted proteins of 56,680 Da that were 70% identical. The 5' nucleotide sequence of groEL1 coded for a protein corresponding to HSP18 that may be a processed gene product. At least two groEL-like genes were present in all 12 Streptomyces species tested; they were not closely linked in the genome. groEL1, but not groEL2, was adjacent to a groES-like gene.

MeSH Terms

Amino Acid Sequence Bacterial Proteins/genetics Base Sequence Chaperonin 60 Cloning, Molecular Genes, Bacterial Heat-Shock Proteins/genetics Molecular Sequence Data Oligonucleotide Probes Plasmids Restriction Mapping Sequence Homology, Nucleic Acid Streptomyces/genetics

Chemicals

Bacterial Proteins Chaperonin 60 Heat-Shock Proteins Oligonucleotide Probes

Authors & Affiliations

4 authors, click to expand affiliations / ORCID

Mazodier P

Unité de Génie Microbiologique, Institut Pasteur, Paris, France.

Guglielmi G

Davies J

Thompson C J

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.173.22.7382-7386.1991

Published

1991-11-00

Pages

7382-6

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC209248

Subset

Databases

GENBANK

M63255, M64328, M64329, M64330, M64331, M64332, M76657, M76658, S64936, S64939

External Links

PubMed source DOI: 10.1128/jb.173.22.7382-7386.1991 PMC Full Text View journal details

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Characterization of the groEL-like genes in Streptomyces albus.

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