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PMID: 168401 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Intracellular synthesis of mouse mammary tumor virus polypeptides: indication of a precursor glycoprotein.

Journal of virology ·Vol. 16 ·No. 2 ·1975-08-00 ·Pages 250-8

Dickson C, Puma JP, Nandi S

Abstract

Mouse mammary tumor virus polypeptides were detected in the cytoplasm of mouse mammary tumor cell cultures using immunological precipitation techniques. The anti-mouse mammary tumor virus serum precipitated the major virion glycoproteins gp49 and gp37.5/33.5 and a viral-related nonvirion glycoprotein of 76,000 daltons. Subcellular fractionation studies revelaed that the cell-associated virion glycoproteins were present in the membrane fraction. Pulsechase experiments indicated that a viral-related nonvirion glycoprotein of 76,000 daltons may be a precursor to one or more of the virion glycoproteins.

MeSH Terms
Adenocarcinoma Animals Cell Fractionation Cell Membrane/metabolism Cell-Free System Culture Techniques Cytoplasm/metabolism Electrophoresis, Polyacrylamide Gel Glucosamine/metabolism Glycoproteins/metabolism Mammary Neoplasms, Experimental Mammary Tumor Virus, Mouse/metabolism Methionine/metabolism Mice Molecular Weight Neoplasm Proteins/metabolism Peptide Biosynthesis Precipitin Tests Protein Precursors/metabolism Sulfur Radioisotopes Tritium Viral Proteins/biosynthesis
Chemicals
Glycoproteins Neoplasm Proteins Protein Precursors Sulfur Radioisotopes Viral Proteins Tritium Methionine Glucosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Dickson C
Puma J P
Nandi S
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27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.16.2.250-258.1975
Published
1975-08-00
Pages
250-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC354661
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.16.2.250-258.1975 PMC Full Text View journal details
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