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PMID: 16845603 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Gene expression profiling in Salmonella Choleraesuis-infected porcine lung using a long oligonucleotide microarray.

Mammalian genome : official journal of the International Mammalian Genome Society ·Vol. 17 ·No. 7 ·2006-07-00 ·Pages 777-89

Zhao SH, Kuhar D, Lunney JK, Dawson H, Guidry C, Uthe JJ, Bearson SM, Recknor J, Nettleton D, Tuggle CK

Abstract

Understanding the transcriptional response to pathogenic bacterial infection within food animals is of fundamental and applied interest. To determine the transcriptional response to Salmonella enterica serovar Choleraesuis (SC) infection, a 13,297-oligonucleotide swine array was used to analyze RNA from control, 24-h postinoculation (hpi), and 48-hpi porcine lung tissue from pigs infected with SC. In total, 57 genes showed differential expression (p < 0.001; false discovery rate = 12%). Quantitative real-time PCR (qRT-PCR) of 61 genes was used to confirm the microarray results and to identify pathways responding to infection. Of the 33 genes identified by microarray analysis as differentially expressed, 23 were confirmed by qRT-PCR results. A novel finding was that two transglutaminase family genes (TGM1 and TGM3) showed dramatic increases in expression postinoculation; combined with several other apoptotic genes, they indicated the induction of apoptotic pathways during SC infection. A predominant T helper 1-type immune response occurred during infection, with interferon gamma (IFNG) significantly increased at 48 hpi. Genes induced by IFNs (GBP1, GBP2, C1S, C1R, MHC2TA, PSMB8, TAP1, TAP2) showed increased expression during porcine lung infection. These data represent the first thorough investigation of gene regulation pathways that control an important porcine respiratory and foodborne bacterial infection.

MeSH Terms
Animals Gene Expression Profiling/veterinary Lung Diseases/genetics,microbiology,veterinary Oligonucleotide Array Sequence Analysis/veterinary Reverse Transcriptase Polymerase Chain Reaction/veterinary Salmonella Infections, Animal/genetics,microbiology Salmonella arizonae Swine Swine Diseases/genetics,microbiology
Authors & Affiliations
10 authors, click to expand affiliations / ORCID
Zhao Shu-Hong
Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, 50011, USA.
Kuhar Daniel
Lunney Joan K
Dawson Harry
Guidry Catherine
Uthe Jolita J
Bearson Shawn M D
Recknor Justin
Nettleton Dan
Tuggle Christopher K
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Article Info
Journal
Mammalian genome : official journal of the International Mammalian Genome Society
Abbr.
Mamm Genome
ISSN
0938-8990
DOI
10.1007/s00335-005-0155-3
Published
2006-07-00
Epub
2006-00-15
Pages
777-89
Language
English
Region
United States
NLM ID
9100916
Subset
IM
External Links
PubMed source DOI: 10.1007/s00335-005-0155-3 View journal details
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