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PMID: 16847351 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

SUMO modifications control assembly of synaptonemal complex and polycomplex in meiosis of Saccharomyces cerevisiae.

Genes & development ·Vol. 20 ·No. 15 ·2006-08-01 ·Pages 2067-81

Cheng CH, Lo YH, Liang SS, Ti SC, Lin FM, Yeh CH, Huang HY, Wang TF

Abstract

The synaptonemal complex (SC) is a proteinaceous complex that apparently mediates synapsis between homologous chromosomes during meiotic prophase. In Saccharomyces cerevisiae, the Zip1 protein is the integral component of the SC. In the absence of a DNA double-strand break or the SC initiation protein Zip3, Zip1 proteins aggregate to form a polycomplex (PC). In addition, Zip1 is also responsible for DSB-independent nonhomologous centromere coupling at early meiotic prophase. We report here that Zip3 is a SUMO (small ubiquitin-related modifier) E3 ligase and that Zip1 is a binding protein for SUMO-conjugated products. Our results also suggest that at early meiotic prophase, Zip1 interacts with Zip3-independent Smt3 conjugates (e.g., Top2) to promote nonhomologous centromere coupling. At and after mid-prophase, the Zip1 protein begins to associate with Zip3-dependent Smt3 conjugates (e.g., Red1) along meiotic chromosomes in the wild-type cell to form SCs and with Smt3 polymeric chains in the zip3 mutant to form PCs.

MeSH Terms
Amino Acid Sequence Centromere/physiology Chromosomes, Fungal Meiosis Molecular Sequence Data Nuclear Proteins Prophase Saccharomyces cerevisiae/genetics,growth & development,metabolism Saccharomyces cerevisiae Proteins/genetics,metabolism Sequence Homology, Amino Acid Small Ubiquitin-Related Modifier Proteins/physiology Synaptonemal Complex/physiology
Chemicals
Nuclear Proteins Saccharomyces cerevisiae Proteins Small Ubiquitin-Related Modifier Proteins Zip1 protein, S cerevisiae
Authors & Affiliations
8 authors, click to expand affiliations / ORCID
Cheng Chung-Hsu
Institute of Biochemical Science, National Taiwan University, Taipei.
Lo Yu-Hui
Liang Shu-Shan
Ti Shih-Chieh
Lin Feng-Ming
Yeh Chia-Hui
Huang Han-Yi
Wang Ting-Fang
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Article Info
Journal
Genes & development
Abbr.
Genes Dev
ISSN
0890-9369
DOI
10.1101/gad.1430406
Published
2006-08-01
Epub
2006-00-17
Pages
2067-81
Language
English
Region
United States
NLM ID
8711660
PMCID
PMC1536058
Subset
IM
External Links
PubMed source DOI: 10.1101/gad.1430406 PMC Full Text View journal details
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