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PMID: 1684878 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity.

Science (New York, N.Y.) ·Vol. 254 ·No. 5039 ·1991-12-20 ·Pages 1814-6

Segil N, Roberts SB, Heintz N

Abstract

Oct-1 is a transcription factor involved in the cell cycle regulation of histone H2B gene transcription and in the transcription of other cellular housekeeping genes. Oct-1 is hyperphosphorylated as cells enter mitosis, and mitosis-specific phosphorylation is reversed as cells exit mitosis. A mitosis-specific phosphorylation site in the homeodomain of Oct-1 was phosphorylated in vitro by protein kinase A. Phosphorylation of this site correlated with inhibition of Oct-1 DNA binding activity in vivo and in vitro. The inhibition of Oct-1 DNA binding during mitosis suggests a mechanism by which the general inhibition of transcription during mitosis might occur.

MeSH Terms

Amino Acid Sequence Animals Base Sequence Cattle Cell Cycle Cloning, Molecular DNA, Neoplasm/metabolism DNA-Binding Proteins/genetics,metabolism Genes, Homeobox HeLa Cells Histones/genetics Host Cell Factor C1 Humans Mitosis Molecular Sequence Data Myocardium/enzymology Octamer Transcription Factor-1 Oligodeoxyribonucleotides Peptide Mapping Phosphopeptides/isolation & purification Phosphorylation Protein Kinases/metabolism Transcription Factors/genetics,metabolism

Chemicals

DNA, Neoplasm DNA-Binding Proteins HCFC1 protein, human Histones Host Cell Factor C1 Octamer Transcription Factor-1 Oligodeoxyribonucleotides POU2F1 protein, human Phosphopeptides Transcription Factors Protein Kinases

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Segil N

Laboratory of Molecular Biology, Howard Hughes Medical Institute, Rockefeller University, New York, NY 10021.

Roberts S B

Heintz N

Article Info

Journal

Science (New York, N.Y.)

Abbr.

Science

ISSN

0036-8075

DOI

10.1126/science.1684878

Published

1991-12-20

Pages

1814-6

Language

English

Region

United States

NLM ID

0404511

Subset

Grants

NIGMS NIH HHS · GM 13752 · United States

NIGMS NIH HHS · GM 32544 · United States

External Links

PubMed source DOI: 10.1126/science.1684878 View journal details

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Mitotic phosphorylation of the Oct-1 homeodomain and regulation of Oct-1 DNA binding activity.

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