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PMID: 168558 Published · ppublish English Journal Article

The 5' end group of tobacco mosaic virus RNA is m7G5' ppp5' Gp.

Nucleic acids research ·Vol. 2 ·No. 7 ·1975-07-00 ·Pages 1189-201

Zimmern D

Abstract

RNA extracted from CsC1-purified virions of tobacco mosaic virus is shown to give rise to an unusual nucleotide on digestion which RNAase T2, in addition to the four major nucleotides. This minor component has the electrophoretic characteristics of a phosphorylated end group, but is partially resistant to bacterial alkaline phosphatase. It is, however, a substrate for venom phosphodiesterase or nucleotide pyrophosphatase, yielding products which imply the structure m7G5'ppp5'Gp. TMV RNA, like many animal cellular and viral mRNAs recently examined, therefore has a 5' terminus blocked by a methylated nucleotide inverted with respect to the rest of the chain.

MeSH Terms
Alkaline Phosphatase Animals Base Sequence Chromatography, Thin Layer Coliphages/enzymology Escherichia coli/enzymology Guanine Nucleotides/analysis Nucleotides Pancreas/enzymology Phosphoric Diester Hydrolases Pyrophosphatases RNA, Viral/analysis,isolation & purification Ribonucleases Snakes Tobacco Mosaic Virus/analysis Venoms
Chemicals
Guanine Nucleotides Nucleotides RNA, Viral Venoms Ribonucleases Alkaline Phosphatase Phosphoric Diester Hydrolases Pyrophosphatases
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Zimmern D
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32 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/2.7.1189
Published
1975-07-00
Pages
1189-201
Language
English
Region
England
NLM ID
0411011
PMCID
PMC343505
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/2.7.1189 PMC Full Text View journal details
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