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PMID: 1688422 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A major immunogenic 36,000-molecular-weight antigen from Mycobacterium leprae contains an immunoreactive region of proline-rich repeats.

Infection and immunity ·Vol. 58 ·No. 1 ·1990-01-00 ·Pages 80-7

Thole JE, Stabel LF, Suykerbuyk ME, De Wit MY, Klatser PR, Kolk AH, Hartskeerl RA

Abstract

The 36,000-molecular-weight antigen (36K antigen) of Mycobacterium leprae is a major immunogenic protein carrying common and specific antigenic determinants recognized by antibodies and T cells in leprosy patients. Recombinant DNA clones containing the complete gene coding for the 36 K antigen, designated in this paper as PRA, were isolated from both lambda gt11 and cosmid libraries of the M. leprae genome. The DNA sequence of the pra gene coded for a polypeptide of 249 amino acids with a predicted molecular mass of 26,299 daltons. The deduced amino acid sequence revealed a proline-rich (42%) amino-terminal region containing a number of repeated sequences similar or identical to the sequence PGGSYPPPPP. The reactivity of four monoclonal antibodies (F47-9, F67-1, F67-5, and F126-5) was directed to this proline-rich region of the PRA protein. DNA sequence and immunological data indicated that the lambda gt11 recombinant Y3180, which was previously isolated by using antibody F47-9 (R. A. Young, V. Mehra, D. Sweetser, T. Buchanan, J. Clark-Curtiss, R. W. Davis, and B. R. Bloom, Nature (London) 316:450-452, 1985), specifies a fusion protein unrelated to PRA but containing a similar epitope recognized by F47-9.

MeSH Terms
Amino Acid Sequence Animals Antibodies, Monoclonal/immunology Antigens, Bacterial/genetics,immunology Base Sequence Cloning, Molecular Epitopes Guinea Pigs Hypersensitivity, Delayed/immunology Molecular Sequence Data Molecular Weight Mycobacterium leprae/genetics,immunology Proline Recombinant Proteins/immunology Restriction Mapping Skin Tests
Chemicals
Antibodies, Monoclonal Antigens, Bacterial Epitopes Recombinant Proteins Proline
Authors & Affiliations
7 authors, click to expand affiliations / ORCID
Thole J E
N. H. Swellengrebel Laboratory of Tropical Hygiene, Royal Tropical Institute, Amsterdam, The Netherlands.
Stabel L F
Suykerbuyk M E
De Wit M Y
Klatser P R
Kolk A H
Hartskeerl R A
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.58.1.80-87.1990
Published
1990-01-00
Pages
80-7
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC258411
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.58.1.80-87.1990 PMC Full Text View journal details
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