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PMID: 1688565 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Stimulation of CD5 enhances signal transduction by the T cell antigen receptor.

The Journal of clinical investigation ·Vol. 85 ·No. 1 ·1990-01-00 ·Pages 130-4

Imboden JB, June CH, McCutcheon MA, Ledbetter JA

Abstract

After the addition of a CD3 monoclonal antibody to peripheral T cells that have been previously stimulated with phytohemagglutinin, inositol phosphates are produced at a rapid rate for 2 min and at a much slower rate thereafter. Stimulation of CD5 allows CD3-mediated production of inositol phosphates to be sustained at a brisk rate for greater than 20 min and augments the initial CD3-mediated increase in inositol trisphosphate and release of intracellular Ca2+. Thus, perturbation of CD5 by monoclonal antibody enhances the ability of the CD3-antigen receptor complex to couple to the inositol phospholipid pathway. This effect of CD5 is independent of any direct effect of the CD5 monoclonal antibody on the levels of inositol phosphates.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, CD/immunology Antigens, Differentiation/immunology CD5 Antigens Cells, Cultured Humans Inositol 1,4,5-Trisphosphate/metabolism Kinetics Lymphocyte Activation Receptors, Antigen, T-Cell/physiology Signal Transduction T-Lymphocytes/immunology,physiology
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Differentiation CD5 Antigens Receptors, Antigen, T-Cell Inositol 1,4,5-Trisphosphate
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Imboden J B
Department of Medicine, University of California, San Francisco.
June C H
McCutcheon M A
Ledbetter J A
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114402
Published
1990-01-00
Pages
130-4
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296396
Subset
IM
Grants
NIAID NIH HHS · R01 AI-26644-01 · United States
External Links
PubMed source DOI: 10.1172/JCI114402 PMC Full Text View journal details
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