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PMID: 1688840 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Loss of 4.5S RNA induces the heat shock response and lambda prophage in Escherichia coli.

Journal of bacteriology ·Vol. 172 ·No. 2 ·1990-02-00 ·Pages 1151-4

Bourgaize DB, Phillips TA, VanBogelen RA, Jones PG, Neidhardt FC, Fournier MJ

Abstract

During depletion of 4.5S RNA, cells of Escherichia coli displayed a heat shock response that was simultaneous with the first detectable effect on ribosome function and before major effects on cell growth. Either 4.5S RNA is involved directly in regulating the heat shock response, or this particular impairment of protein synthesis uniquely induces the heat shock response. Several hours later, lambda prophage was induced and the cells lysed.

MeSH Terms
Bacteriophage lambda/drug effects,physiology Electrophoresis, Gel, Two-Dimensional Escherichia coli/drug effects,growth & development,physiology Heat-Shock Proteins/biosynthesis,isolation & purification Hot Temperature Isopropyl Thiogalactoside/pharmacology Kinetics Lysogeny Methionine/metabolism RNA, Bacterial/physiology
Chemicals
Heat-Shock Proteins RNA, Bacterial Isopropyl Thiogalactoside Methionine
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Bourgaize D B
Department of Biochemistry, University of Massachusetts, Amherst 01003.
Phillips T A
VanBogelen R A
Jones P G
Neidhardt F C
Fournier M J
References (19)
19 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.172.2.1151-1154.1990
Published
1990-02-00
Pages
1151-4
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC208552
Subset
IM
Grants
NIGMS NIH HHS · GM17892 · United States
NIGMS NIH HHS · GM19351 · United States
External Links
PubMed source DOI: 10.1128/jb.172.2.1151-1154.1990 PMC Full Text View journal details
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