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PMID: 16892052 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't

Smc5-Smc6 mediate DNA double-strand-break repair by promoting sister-chromatid recombination.

Nature cell biology ·Vol. 8 ·No. 9 ·2006-09-00 ·Pages 1032-4

De Piccoli G, Cortes-Ledesma F, Ira G, Torres-Rosell J, Uhle S, Farmer S, Hwang JY, Machin F, Ceschia A, McAleenan A, Cordon-Preciado V, Clemente-Blanco A, Vilella-Mitjana F, Ullal P, Jarmuz A, Leitao B, Bressan D, Dotiwala F, Papusha A, Zhao X, Myung K, Haber JE, Aguilera A, Aragón L

Abstract

DNA double-strand breaks (DSB) can arise during DNA replication, or after exposure to DNA-damaging agents, and their correct repair is fundamental for cell survival and genomic stability. Here, we show that the Smc5-Smc6 complex is recruited to DSBs de novo to support their repair by homologous recombination between sister chromatids. In addition, we demonstrate that Smc5-Smc6 is necessary to suppress gross chromosomal rearrangements. Our findings show that the Smc5-Smc6 complex is essential for genome stability as it promotes repair of DSBs by error-free sister-chromatid recombination (SCR), thereby suppressing inappropriate non-sister recombination events.

MeSH Terms
Cell Cycle Proteins/physiology DNA/metabolism DNA Damage Deoxyribonucleases, Type II Site-Specific/metabolism Genomic Instability Saccharomyces cerevisiae/genetics,physiology Saccharomyces cerevisiae Proteins/physiology Sister Chromatid Exchange
Chemicals
Cell Cycle Proteins SMC5 protein, S cerevisiae SMC6 protein, S cerevisiae Saccharomyces cerevisiae Proteins DNA SCEI protein, S cerevisiae Deoxyribonucleases, Type II Site-Specific
Authors & Affiliations
24 authors, click to expand affiliations / ORCID
De Piccoli Giacomo
Cell Cycle Group, MRC Clinical Sciences Centre, Imperial College London, Du Cane Road, London W12 0NN, UK.
Cortes-Ledesma Felipe
Ira Gregory
Torres-Rosell Jordi
Uhle Stefan
Farmer Sarah
Hwang Ji-Young
Machin Felix
Ceschia Audrey
McAleenan Alexandra
Cordon-Preciado Violeta
Clemente-Blanco Andrés
Vilella-Mitjana Felip
Ullal Pranav
Jarmuz Adam
Leitao Beatriz
Bressan Debra
Dotiwala Farokh
Papusha Alma
Zhao Xiaolan
Myung Kyungjae
Haber James E
Aguilera Andrés
Aragón Luis
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Article Info
Journal
Nature cell biology
Abbr.
Nat Cell Biol
ISSN
1465-7392
DOI
10.1038/ncb1466
Published
2006-09-00
Epub
2006-00-06
Pages
1032-4
Language
English
Region
England
NLM ID
100890575
PMCID
PMC4493748
Subset
IM
Grants
NIGMS NIH HHS · GM20056 · United States
Medical Research Council · MC_U120074328 · United Kingdom
NIGMS NIH HHS · R01 GM080600 · United States
NIGMS NIH HHS · R37 GM020056 · United States
NIGMS NIH HHS · R01 GM020056 · United States
External Links
PubMed source DOI: 10.1038/ncb1466 PMC Full Text View journal details
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