Home LiteratureArticle Details
PMID: 1689762 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Cytotoxic T cells recognize a peptide from the circumsporozoite protein on malaria-infected hepatocytes.

The Journal of experimental medicine ·Vol. 171 ·No. 3 ·1990-03-01 ·Pages 763-73

Weiss WR, Mellouk S, Houghten RA, Sedegah M, Kumar S, Good MF, Berzofsky JA, Miller LH, Hoffman SL

Abstract

Irradiated malaria sporozoites can induce CD8+ T cells that are required for protection against infection. However, the parasite antigens targeted by this immune response are unknown. We have discovered a 16-amino acid epitope from the Plasmodium yoelii circumsporozoite (CS) protein that is recognized by cytotoxic T cells from immune mice. Lymphocytes stimulated with this peptide can kill P. yoelii liver stage parasites in vitro in an MHC-restricted, antigen-specific manner. Thus, epitopes from the CS protein are presented on the surface of infected hepatocytes and can be targets for T cells, even though intact CS protein has not been detected on the surface of the infected hepatocyte. A vaccine that induced CTL to parasite antigens might protect humans against malaria by eliminating liver stage parasites.

MeSH Terms
Animals Epitopes/analysis Female Immunization Liver/immunology,parasitology Malaria/immunology Mice Mice, Inbred BALB C Peptide Fragments/immunology Plasmodium yoelii/immunology Protozoan Proteins/immunology T-Lymphocytes, Cytotoxic/immunology Vaccines/immunology
Chemicals
Epitopes Peptide Fragments Protozoan Proteins Vaccines
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Weiss W R
Infectious Disease Department, Naval Medical Research Institute, Bethesda, Maryland 20814.
Mellouk S
Houghten R A
Sedegah M
Kumar S
Good M F
Berzofsky J A
Miller L H
Hoffman S L
References (30)
30 references, click to expand
  1. Serological reactivity of in vitro cultured exoerythrocytic stages of Plasmodium berghei in indirect immunofluorescent or immunoperoxidase antibody tests.
    Am J Trop Med Hyg. 1983 Jan;32(1):24-30 PMID: 6337518
  2. IgG or IgM monoclonal antibodies reactive with different determinants on the molecular complex bearing Lyt 2 antigen block T cell-mediated cytolysis in the absence of complement.
    J Immunol. 1980 Dec;125(6):2665-72 PMID: 6159417
  3. Immunization of man against sporozite-induced falciparum malaria.
    Am J Med Sci. 1973 Sep;266(3):169-77 PMID: 4583408
  4. Protective immunity produced by the injection of x-irradiated sporozoites of plasmodium berghei.
    Nature. 1967 Oct 14;216(5111):160-2 PMID: 6057225
  5. Structure of the gene encoding the circumsporozoite protein of Plasmodium yoelii. A rodent model for examining antimalarial sporozoite vaccines.
    J Biol Chem. 1987 Mar 5;262(7):2937-40 PMID: 3102479
  6. Class I major histocompatibility complex-restricted cytolytic T lymphocytes recognize a limited number of sites on the influenza hemagglutinin.
    Proc Natl Acad Sci U S A. 1989 Jan;86(1):277-81 PMID: 2463623
  7. CD8+ T cells (cytotoxic/suppressors) are required for protection in mice immunized with malaria sporozoites.
    Proc Natl Acad Sci U S A. 1988 Jan;85(2):573-6 PMID: 2963334
  8. Structural model of HLA-DR1 restricted T cell antigen recognition.
    Cell. 1988 Feb 26;52(4):515-23 PMID: 2963699
  9. Gamma interferon, CD8+ T cells and antibodies required for immunity to malaria sporozoites.
    Nature. 1987 Dec 17-23;330(6149):664-6 PMID: 3120015
  10. The fate of the circumsporozoite antigens during the exoerythrocytic stage of Plasmodium berghei.
    Eur J Cell Biol. 1988 Apr;46(1):25-30 PMID: 3294008
  11. Cloned cytotoxic T cells recognize an epitope in the circumsporozoite protein and protect against malaria.
    Nature. 1989 Sep 28;341(6240):323-6 PMID: 2477703
  12. Cytotoxic T cells specific for the circumsporozoite protein of Plasmodium falciparum.
    Nature. 1988 Jul 21;334(6179):258-60 PMID: 2456467
  13. A malaria T-cell epitope recognized in association with most mouse and human MHC class II molecules.
    Nature. 1988 Dec 22-29;336(6201):778-80 PMID: 2462673
  14. An immunodominant epitope of the human immunodeficiency virus envelope glycoprotein gp160 recognized by class I major histocompatibility complex molecule-restricted murine cytotoxic T lymphocytes.
    Proc Natl Acad Sci U S A. 1988 May;85(9):3105-9 PMID: 2452443
  15. Human T-cell recognition of the circumsporozoite protein of Plasmodium falciparum: immunodominant T-cell domains map to the polymorphic regions of the molecule.
    Proc Natl Acad Sci U S A. 1988 Feb;85(4):1199-203 PMID: 2448793
  16. Sequence variation in putative functional domains of the circumsporozoite protein of Plasmodium falciparum. Implications for vaccine development.
    J Biol Chem. 1987 Sep 5;262(25):11935-9 PMID: 2442154
  17. Construction of synthetic immunogen: use of new T-helper epitope on malaria circumsporozoite protein.
    Science. 1987 Feb 27;235(4792):1059-62 PMID: 2434994
  18. General method for the rapid solid-phase synthesis of large numbers of peptides: specificity of antigen-antibody interaction at the level of individual amino acids.
    Proc Natl Acad Sci U S A. 1985 Aug;82(15):5131-5 PMID: 2410914
  19. Sporozoite vaccine induces genetically restricted T cell elimination of malaria from hepatocytes.
    Science. 1989 Jun 2;244(4908):1078-81 PMID: 2524877
  20. Genetic control of immunity to Plasmodium yoelii sporozoites.
    J Immunol. 1989 Dec 15;143(12):4263-6 PMID: 2512354
  21. Immune response gene regulation of immunity to Plasmodium berghei sporozoites and circumsporozoite protein vaccines. Overcoming genetic restriction with whole organism and subunit vaccines.
    J Immunol. 1989 May 15;142(10):3581-4 PMID: 2497175
  22. Expression of Plasmodium berghei circumsporozoite antigen on the surface of exoerythrocytic schizonts and merozoites.
    Am J Trop Med Hyg. 1989 Jul;41(1):9-17 PMID: 2669544
  23. Cultivation of the exoerythrocytic stage of Plasmodium berghei in primary cultures of mouse hepatocytes and continuous mouse cell lines.
    In Vitro Cell Dev Biol. 1989 Sep;25(9):857-62 PMID: 2676959
  24. Oral Salmonella typhimurium vaccine expressing circumsporozoite protein protects against malaria.
    Science. 1988 Apr 15;240(4850):336-8 PMID: 3281260
  25. Murine cytotoxic T lymphocyte recognition of individual influenza virus proteins. High frequency of nonresponder MHC class I alleles.
    J Exp Med. 1988 Nov 1;168(5):1935-9 PMID: 3263469
  26. A liver-stage-specific antigen of Plasmodium falciparum characterized by gene cloning.
    Nature. 1987 Sep 10-16;329(6135):164-7 PMID: 3306406
  27. Plasmodium berghei: cloning of the circumsporozoite protein gene.
    Exp Parasitol. 1987 Jun;63(3):295-300 PMID: 3556207
  28. Effect of antibodies to recombinant and synthetic peptides on P. falciparum sporozoites in vitro.
    Science. 1986 Jan 10;231(4734):156-9 PMID: 3510455
  29. Hydrophobicity scales and computational techniques for detecting amphipathic structures in proteins.
    J Mol Biol. 1987 Jun 5;195(3):659-85 PMID: 3656427
  30. [In vitro infestation of adult thamnomys hepatocytes with Plasmodium yoelii sporozoites; schizogony and release of infecting merozoites].
    C R Seances Acad Sci III. 1982 May 24;294(19):963-5 PMID: 6814716
Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.171.3.763
Published
1990-03-01
Pages
763-73
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187765
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.171.3.763 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2