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PMID: 1689763 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Human anti-p68 autoantibodies recognize a common epitope of U1 RNA containing small nuclear ribonucleoprotein and influenza B virus.

The Journal of experimental medicine ·Vol. 171 ·No. 3 ·1990-03-01 ·Pages 819-29

Guldner HH, Netter HJ, Szostecki C, Jaeger E, Will H

Abstract

Autoantibodies from patients with systemic rheumatic diseases were used to map antigenic sites on the 68-kD autoantigen (p68) associated with (U1)RNA-containing small nuclear ribonucleoprotein (snRNP) particles. With truncated recombinant fusion proteins and synthetic peptides, a subset of anti-p68 autoantibodies was found to recognize the amino acid sequence motif Glu-Arg-Lys-Arg-Arg (ERKRR). To investigate the possible involvement of epitopes shared by microbial antigens and host self-components in initiation of autoimmunity (molecular mimicry), a sequence data bank was screened for proteins containing an amino acid motif identical or related to ERKRR. The identical motif was found on the M1 matrix protein of influenza B viruses, and affinity-purified human anti-ERKRR autoantibodies recognized this epitope also in the viral amino acid sequence context. The common epitope recognized by human autoantibodies suggests that influenza B virus infection may play a role in initiation of the anti-p68 and anti-(U1)RNP autoimmune response.

MeSH Terms
Animals Autoantibodies/immunology Cross Reactions Epitopes/analysis Female Humans Immunization Influenza B virus/immunology Mice Mice, Inbred BALB C Ribonucleoproteins/immunology Ribonucleoproteins, Small Nuclear Viral Matrix Proteins/immunology
Chemicals
Autoantibodies Epitopes Ribonucleoproteins Ribonucleoproteins, Small Nuclear Viral Matrix Proteins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Guldner H H
Max-Planck-Institut für Biochemie, Martinsried, Federal Republic of Germany.
Netter H J
Szostecki C
Jaeger E
Will H
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.171.3.819
Published
1990-03-01
Pages
819-29
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2187766
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.171.3.819 PMC Full Text View journal details
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