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PMID: 1690821 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

An early postinfection signal mediated by monoclonal anti-beta 2 microglobulin antibody is responsible for delayed production of human immunodeficiency virus type 1 in peripheral blood mononuclear cells.

Journal of virology ·Vol. 64 ·No. 4 ·1990-04-00 ·Pages 1459-64

Corbeau P, Devaux C, Kourilsky F, Chermann JC

Abstract

We recently found (C. Devaux, J. Boucraut, G. Poirier, P. Corbeau, F. Rey, M. Benkirane, B. Perarneau, F. Kourilsky, and J.C. Chermann, submitted for publication) a latency in the human immunodeficiency virus (HIV) type 1 cytopathic effect in the human T-cell lymphotropic virus type I immortalized T-cell line MT4 that was mediated by anti-beta 2 microglobulin (beta 2m) monoclonal antibodies (MAb). Here we describe a delay in viral particle production in peripheral blood mononuclear cells (PBMC) that was mediated by three (B1-1G6, B2-62-2, and HC11-151-1) of four anti-beta 2m MAb tested, the nonefficient MAb (C21-48A) being specific for an epitope on beta 2m that was masked by association with the human leukocyte antigen class I heavy chain. Experiments were designed to determine the mechanism of interference. PBMC incubated with anti-beta 2m MAb before viral exposure were not protected from HIV infection. In addition, anti-beta 2m MAb were not efficient in preventing syncytium formation between HIV-infected PBMC and CD4-positive MT4 cells. In contrast, anti-beta 2m MAb treatment of freshly infected PBMC significantly delayed HIV production in these cells. The window of cell sensitivity to anti-beta 2m MAb treatment took place during a very early post-HIV-binding stage. The possible mechanism of anti-beta 2m MAb action is discussed.

MeSH Terms
Acquired Immunodeficiency Syndrome/immunology Antibodies, Monoclonal/immunology Cell Line Cytopathogenic Effect, Viral/immunology Giant Cells/immunology HIV-1/enzymology,growth & development Humans Leukocytes, Mononuclear/immunology,microbiology RNA-Directed DNA Polymerase/metabolism Time Factors Virus Replication/immunology beta 2-Microglobulin/immunology
Chemicals
Antibodies, Monoclonal beta 2-Microglobulin RNA-Directed DNA Polymerase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Corbeau P
Unité de Recherches sur les Rétrovirus et Maladies Associées, Institut National de la Santé, Marseille, France.
Devaux C
Kourilsky F
Chermann J C
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.64.4.1459-1464.1990
Published
1990-04-00
Pages
1459-64
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC249279
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.64.4.1459-1464.1990 PMC Full Text View journal details
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