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PMID: 1691706 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

The optional E. coli prr locus encodes a latent form of phage T4-induced anticodon nuclease.

The EMBO journal ·Vol. 9 ·No. 5 ·1990-05-00 ·Pages 1383-9

Levitz R, Chapman D, Amitsur M, Green R, Snyder L, Kaufmann G

Abstract

The optional Escherichia coli prr locus restricts phage T4 mutants lacking polynucleotide kinase or RNA ligase. Underlying this restriction is the specific manifestation of the T4-induced anticodon nuclease, an enzyme which triggers the cleavage-ligation of the host tRNALys. We report here the molecular cloning, nucleotide sequence and mutational analysis of prr-associated DNA. The results indicate that prr encodes a latent form of anticodon nuclease consisting of a core enzyme and cognate masking agents. They suggest that the T4-encoded factors of anticodon nuclease counteract the prr-encoded masking agents, thus activating the latent enzyme. The encoding of a tRNA cleavage-ligation pathway by two separate genetic systems which cohabitate E. coli may provide a clue to the evolution of RNA splicing mechanisms mediated by proteins.

MeSH Terms
Amino Acid Sequence Base Sequence Cloning, Molecular DNA Mutational Analysis Enzyme Activation/genetics Escherichia coli/enzymology,genetics Gene Expression Regulation, Bacterial/genetics Molecular Sequence Data RNA Splicing/genetics RNA, Bacterial/metabolism Ribonucleases/genetics,metabolism T-Phages/genetics
Chemicals
RNA, Bacterial Ribonucleases anticodon nuclease
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Levitz R
Department of Biochemistry, Tel Aviv University, Ramat Aviv, Israel.
Chapman D
Amitsur M
Green R
Snyder L
Kaufmann G
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Article Info
Journal
The EMBO journal
Abbr.
EMBO J
ISSN
0261-4189
Published
1990-05-00
Pages
1383-9
Language
English
Region
England
NLM ID
8208664
PMCID
PMC551823
Subset
IM
Databases
GENBANK
X52284
External Links
PubMed source PMC Full Text View journal details
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