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PMID: 16920793 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Side population in adult murine epidermis exhibits phenotypic and functional characteristics of keratinocyte stem cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 103 ·No. 35 ·2006-08-29 ·Pages 13168-73

Redvers RP, Li A, Kaur P

Abstract

Based on functional studies in the bone marrow, it has been suggested that the ability to efflux Hoechst 33342 may represent a universal stem cell trait. In this phenotypic and functional characterization of the Hoechst side population (SP) in adult murine epidermis, we demonstrate that these cells are a rare subset of the keratinocyte stem cell-enriched alpha(6)(bri)CD71(dim) fraction comprising SSC(low)/K14(+)/CD34(-)/Oil red O(-)/c-kit(-)/CD45(-) keratinocytes. Epidermal SPs have the smallest cell and nuclear size but exhibit the highest nuclear-to-cytoplasmic ratio of any fraction examined, consistent with a primitive cell type. Although SPs demonstrated poor cumulative in vitro proliferative output, they exhibited sustained epidermal tissue-regenerative activity in vivo compared with unfractionated and non-SP cells. Collectively, these results indicate that the epidermal SP contains the most potent keratinocyte stem cell population in skin epithelium.

MeSH Terms
Animals Antigens, CD/metabolism Cell Nucleus/metabolism Cell Proliferation Cell Size Cytoplasm/metabolism DNA-Binding Proteins/genetics Epidermal Cells Gene Expression Regulation Homeodomain Proteins/genetics Keratinocytes/cytology Mice Mice, Inbred C57BL Mitosis Nanog Homeobox Protein Octamer Transcription Factor-3/genetics Phenotype RNA, Messenger/genetics,metabolism Rats Rats, Sprague-Dawley Receptors, Transferrin/metabolism Regeneration Stem Cells/cytology Tail/cytology Trachea/cytology
Chemicals
Antigens, CD CD71 antigen DNA-Binding Proteins Homeodomain Proteins Nanog Homeobox Protein Nanog protein, mouse Octamer Transcription Factor-3 Pou5f1 protein, mouse RNA, Messenger Receptors, Transferrin
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Redvers Richard P
Epithelial Stem Cell Biology Laboratory, Peter MacCallum Cancer Centre, Trescowthick Research Laboratories, St. Andrew's Place, East Melbourne, Victoria 3002, Australia.
Li Amy
Kaur Pritinder
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.0602579103
Published
2006-08-29
Epub
2006-00-18
Pages
13168-73
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC1559771
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.0602579103 PMC Full Text View journal details
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