Home LiteratureArticle Details
PMID: 169225 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Pyrimidine biosynthetic pathway of Baccillus subtilis.

Journal of bacteriology ·Vol. 123 ·No. 2 ·1975-08-00 ·Pages 604-15

Potvin BW, Kelleher RJ, Gooder H

Abstract

Biochemical and genetic data were obtained from a series of 51 Pyr- strains of Bacillus subtilis. The observed enzymatic deficiencies allowed the mutants to be placed into 12 clases, some of which represent defects in more than one of the six known pyrimidine biosynthetic enzymes. Mapping analysis by transformation has shown that all the Pyr- mutations are located in a single small area of the B. subtilis genome. A correlation of the biochemical defects and the genetic data has been made. Those mutations conferring similar enzymatic deficiencies were found to be contiguous on the B. subtilis map. Regulatory aspects of the pyrimidine pathway have also been investigated and are compared to previously reported results from other organisms. Evidence is presented which bears upon the possible physical association of the first three enzymes and the association of at least some of the enzymes of this pathway with particulate elements of the cell. A model for the organization of the enzymes is presented with dihydroorotate dehydrogenase as the central enzyme in a proposed aggregate.

MeSH Terms
Aspartate Carbamoyltransferase/metabolism Bacillus subtilis/enzymology,growth & development Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)/metabolism Carboxy-Lyases/metabolism Carboxylic Acids Chromosome Mapping Enzyme Activation Enzyme Induction Enzyme Repression Genes Genetic Linkage Models, Biological Mutation Orotic Acid/analogs & derivatives,biosynthesis,metabolism Oxidoreductases/metabolism Pentosyltransferases/metabolism Pyrimidines/biosynthesis Pyrophosphatases/metabolism Ribose-Phosphate Pyrophosphokinase/metabolism Transformation, Genetic Uracil Nucleotides
Chemicals
Carboxylic Acids Pyrimidines Uracil Nucleotides Orotic Acid Oxidoreductases Aspartate Carbamoyltransferase Pentosyltransferases Ribose-Phosphate Pyrophosphokinase Pyrophosphatases Carboxy-Lyases Carbamoyl-Phosphate Synthase (Glutamine-Hydrolyzing)
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Potvin B W
Kelleher R J
Gooder H
References (36)
36 references, click to expand
  1. Genetic mapping of aro, pyr, and pur markers in Klebsiella pneumoniae.
    Jpn J Microbiol. 1971 Jan;15(1):11-20 PMID: 4927838
  2. Pyrimidine metabolism in microorganisms.
    Bacteriol Rev. 1970 Sep;34(3):278-343 PMID: 4919542
  3. Sequence of enzyme synthesis and gene replication during the cell cycle of Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1965 Jul;54(1):64-70 PMID: 4955131
  4. Genetic mapping in Bacillus subtilis.
    J Mol Biol. 1967 Jul 14;27(1):163-85 PMID: 4962145
  5. Chromosome replication in Bacillus subtilis cultures growing at different rates.
    Proc Natl Acad Sci U S A. 1967 Jan;57(1):95-101 PMID: 4963254
  6. Biochemical studies of bacterial sporulation and germination. 8. Patterns of enzyme development during growth and sporulation of Baccillus subtilis.
    J Biol Chem. 1968 Sep 25;243(18):4653-60 PMID: 4971658
  7. Control of pyrimidine biosynthesis in Pseudomonas aeruginosa.
    J Bacteriol. 1968 Nov;96(5):1732-41 PMID: 4973129
  8. Copurification of carbamoyl phosphate synthetase and aspartate transcarbamoylase from mouse spleen.
    Biochem Biophys Res Commun. 1971 Aug 20;44(4):981-8 PMID: 5125237
  9. Initial steps in pyrimidine synthesis in Ehrlich ascites carcinoma.
    Biochem Biophys Res Commun. 1971 Nov 5;45(3):796-802 PMID: 5166848
  10. Genetic analysis of pyrimidine mutants of Salmonella typhimurium.
    Genetics. 1965 Sep;52(3):643-51 PMID: 5324718
  11. Aspartate carbamoyltransferase from rat liver.
    Biochem J. 1966 Oct;101(1):63-9 PMID: 5339547
  12. Modified methods for the determination of carbamyl aspartate.
    Anal Biochem. 1969 Dec;32(3):408-19 PMID: 5361395
  13. Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae.
    J Bacteriol. 1968 Mar;95(3):824-32 PMID: 5651325
  14. Purification and kinetic properties of brain orotidine 5'-phosphate decarboxylase.
    J Biol Chem. 1968 Jul 25;243(14):3924-9 PMID: 5661716
  15. AN ATTEMPT TO FIND PYRIMIDINE INHIBITORS OF A MAMMALIAN ASPARTATE CARBAMOYLTRANSFERASE.
    Biochim Biophys Acta. 1964 May 4;85:338-41 PMID: 14212982
  16. PURIFICATION AND PROPERTIES OF OROTIC ACID-DECARBOXYLATING ENZYMES FROM CALF THYMUS.
    J Biol Chem. 1964 Dec;239:4245-9 PMID: 14247677
  17. Enzymatic synthesis of pyrimidine nucleotides; orotidine-5'-phosphate and uridine-5'-phosphate.
    J Biol Chem. 1955 Jul;215(1):403-51 PMID: 14392174
  18. The enzymology of control by feedback inhibition.
    J Biol Chem. 1962 Mar;237:891-6 PMID: 13897943
  19. Coordination of the synthesis of the enzymes in the pyrimidine pathway of E. coli.
    J Mol Biol. 1962 Dec;5:618-34 PMID: 13970180
  20. END-PRODUCT INHIBITION OF ASPARTATE TRANSCARBAMYLASE IN VARIOUS SPECIES.
    Arch Biochem Biophys. 1964 Mar;104:438-47 PMID: 14161013
  21. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  22. A spectrophotometric method for measuring the breakdown of hydrogen peroxide by catalase.
    J Biol Chem. 1952 Mar;195(1):133-40 PMID: 14938361
  23. Carbamyl phosphate synthesis in Bacillus subtilis.
    Biochem Genet. 1975 Feb;13(1-2):125-43 PMID: 237505
  24. Inactivation of aspartic transcarbamylase in sporulating Bacillus subtilis: demonstration of a requirement for metabolic energy.
    J Bacteriol. 1973 May;114(2):517-27 PMID: 4196242
  25. Genetics and biochemistry of pyrimidine biosynthesis in Bacillus subtilis: linkage between mutations resulting in a requirement for uracil.
    J Bacteriol. 1973 Nov;116(2):577-81 PMID: 4200850
  26. Molecular size and feedback-regulation characteristics of bacterial asartate transcarbamulases.
    Arch Biochem Biophys. 1969 Nov;134(2):352-65 PMID: 4311178
  27. Specific repression of phosphoribosylpyrophosphate synthetase by uridine compounds in Salmonella typhimurium.
    J Bacteriol. 1972 Apr;110(1):450-1 PMID: 4336112
  28. Isolation and partial characterization of regulatory mutants of the pyrimidine pathway in Salmonella typhimurium.
    J Bacteriol. 1972 Mar;109(3):1085-96 PMID: 4551742
  29. A genetic analysis by transformation of a group of uracil-requiring mutants of Diplococcus pneumoniae.
    Genetics. 1969 Jan;61(1):41-60 PMID: 4389852
  30. Uridylic acid synthesis in Ehrlich ascites carcinoma. Properties, subcellular distribution, and nature of enzyme complexes of the six biosynthetic enzymes.
    Biochemistry. 1973 Oct 9;12(21):4039-51 PMID: 4355548
  31. Linkage map of Escherichia coli strain K-12.
    Bacteriol Rev. 1972 Dec;36(4):504-24 PMID: 4568762
  32. Linkage map of Salmonella typhimurium, edition IV.
    Bacteriol Rev. 1972 Dec;36(4):558-86 PMID: 4568764
  33. Dihydro-orotate dehydrogenase of Escherichia coli K12: effects of triton X-100 and phospholipids.
    Biochim Biophys Acta. 1973 Apr 12;302(2):205-15 PMID: 4572995
  34. Dihydro-orotate oxidase of Escherichia coli K12: purification, properties, and relation to the cytoplasmic membrane.
    Biochim Biophys Acta. 1974 Oct 17;364(2):218-32 PMID: 4607848
  35. Genetic analysis and genome structure in Streptomyces coelicolor.
    Bacteriol Rev. 1967 Dec;31(4):373-403 PMID: 4866846
  36. Regulation of pyrimidine and arginine biosynthesis in mammals.
    Adv Enzyme Regul. 1970;9:19-49 PMID: 4941903
Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.123.2.604-615.1975
Published
1975-08-00
Pages
604-15
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC235766
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.123.2.604-615.1975 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2