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PMID: 16926515 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

A rapid method for protein extraction from fission yeast.

Bioscience, biotechnology, and biochemistry ·Vol. 70 ·No. 8 ·2006-08-00 ·Pages 1992-4

Matsuo Y, Asakawa K, Toda T, Katayama S

Abstract

Researchers working with fission yeast conduct protein extraction widely and frequently, but this includes the handling of glass beads, and hence is laborious and cumbersome, especially when dealing with a large number of samples. Here we describe a rapid and reliable method for preparing protein extract from fission yeast, one which is applicable to routine western blotting.

MeSH Terms
Proteins/chemistry,isolation & purification Schizosaccharomyces/chemistry,cytology Sodium Hydroxide/chemistry Time Factors
Chemicals
Proteins Sodium Hydroxide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Matsuo Yuzy
Analytical Research Center for Experimental Sciences, Saga University, Japan.
Asakawa Kazuhide
Toda Takashi
Katayama Satoshi
Article Info
Journal
Bioscience, biotechnology, and biochemistry
Abbr.
Biosci Biotechnol Biochem
ISSN
0916-8451
Published
2006-08-00
Pages
1992-4
Language
English
Region
England
NLM ID
9205717
Subset
IM
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