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PMID: 1693380 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Identification of surface proteins mediating adherence of CD11/CD18-deficient lymphoblastoid cells to cultured human endothelium.

The Journal of clinical investigation ·Vol. 85 ·No. 6 ·1990-06-00 ·Pages 2019-22

Schwartz BR, Wayner EA, Carlos TM, Ochs HD, Harlan JM

Abstract

Patients with the severe form of leukocyte adhesion deficiency syndrome do not express the CD11/CD18 adhesion complex on any of their leukocytes. Nevertheless, their lymphocytes, unlike their phagocytes, emigrate to extravascular sites of inflammation, demonstrating that surface proteins other than CD11/CD18 can mediate lymphocyte adherence to endothelium. Using a B-lymphoblastoid cell line (B-LCL) established from a CD11/CD18-deficient patient and cultured human umbilical vein endothelial cells (HEC), we investigated the CD11/CD18-independent mechanism(s) of lymphocyte adherence to endothelium. Monoclonal antibodies directed to the alpha 4 polypeptide (CD49d) and the beta 1 polypeptide (CD29) of the lymphocyte VLA-4 integrin receptor (CD49d/CD29), and to vascular cell adhesion molecule-1 (VCAM-1) on the endothelial cell significantly inhibited the adherence of the CD11/CD18-deficient B-LCL to untreated HEC and to HEC treated with recombinant human tumor necrosis factor-alpha. We suggest that the interaction of the lymphocyte receptor VLA-4 with the endothelial ligand VCAM-1 induced by cytokines at sites of inflammation or immune reaction represents a CD11/CD18-independent pathway of lymphocyte emigration.

MeSH Terms
Antibodies, Monoclonal/immunology Antigens, CD/immunology Antigens, Differentiation/deficiency,immunology CD11 Antigens CD18 Antigens Cell Adhesion Cell Adhesion Molecules/physiology Cells, Cultured Endothelium, Vascular/cytology,physiology Humans Integrin beta1 Leukocyte-Adhesion Deficiency Syndrome Lymphocytes/physiology Receptors, Very Late Antigen/immunology
Chemicals
Antibodies, Monoclonal Antigens, CD Antigens, Differentiation CD11 Antigens CD18 Antigens Cell Adhesion Molecules Integrin beta1 Receptors, Very Late Antigen
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Schwartz B R
Department of Medicine, University of Washington, Seattle 98195.
Wayner E A
Carlos T M
Ochs H D
Harlan J M
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI114668
Published
1990-06-00
Pages
2019-22
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC296673
Subset
IM
Grants
NHLBI NIH HHS · HL18645 · United States
PHS HHS · IA07073 · United States
NCRR NIH HHS · RR37 · United States
External Links
PubMed source DOI: 10.1172/JCI114668 PMC Full Text View journal details
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