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PMID: 16935876 Published · ppublish English Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Review

A dynamic model for replication protein A (RPA) function in DNA processing pathways.

Nucleic acids research ·Vol. 34 ·No. 15 ·2006-00-00 ·Pages 4126-37

Fanning E, Klimovich V, Nager AR

Abstract

Processing of DNA in replication, repair and recombination pathways in cells of all organisms requires the participation of at least one major single-stranded DNA (ssDNA)-binding protein. This protein protects ssDNA from nucleolytic damage, prevents hairpin formation and blocks DNA reannealing until the processing pathway is successfully completed. Many ssDNA-binding proteins interact physically and functionally with a variety of other DNA processing proteins. These interactions are thought to temporally order and guide the parade of proteins that 'trade places' on the ssDNA, a model known as 'hand-off', as the processing pathway progresses. How this hand-off mechanism works remains poorly understood. Recent studies of the conserved eukaryotic ssDNA-binding protein replication protein A (RPA) suggest a novel mechanism by which proteins may trade places on ssDNA by binding to RPA and mediating conformation changes that alter the ssDNA-binding properties of RPA. This article reviews the structure and function of RPA, summarizes recent studies of RPA in DNA replication and other DNA processing pathways, and proposes a general model for the role of RPA in protein-mediated hand-off.

MeSH Terms
DNA/metabolism DNA Replication/physiology DNA, Single-Stranded/metabolism DNA-Binding Proteins/physiology Models, Biological Protein Conformation Replication Protein A/chemistry,physiology Saccharomyces cerevisiae/genetics
Chemicals
DNA, Single-Stranded DNA-Binding Proteins Replication Protein A DNA
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Fanning Ellen
Department of Biological Sciences, Vanderbilt University, VU Station B 351634, Nashville, TN 37235-1634, USA. [email protected]
Klimovich Vitaly
Nager Andrew R
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
1362-4962
DOI
10.1093/nar/gkl550
Email
[email protected]
Published
2006-00-00
Epub
2006-00-25
Pages
4126-37
Language
English
Region
England
NLM ID
0411011
PMCID
PMC1616954
Subset
IM
Grants
NIGMS NIH HHS · R01 GM052948 · United States
NIGMS NIH HHS · GM52948 · United States
External Links
PubMed source DOI: 10.1093/nar/gkl550 PMC Full Text View journal details
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