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PMID: 1693621 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Subunit selectivity and epitope characterization of mAbs directed against the GABAA/benzodiazepine receptor.

The Journal of cell biology ·Vol. 110 ·No. 6 ·1990-06-00 ·Pages 2043-8

Ewert M, Shivers BD, Lüddens H, Möhler H, Seeburg PH

Abstract

mAbs bd 17, bd 24, and bd 28 raised against bovine cerebral gamma-aminobutyric acid (GABAA)/benzodiazepine receptors were analyzed for their ability to detect each of 12 GABAA receptor subunits expressed in cultured mammalian cells. Results showed that mAb bd 17 recognizes epitopes on both beta 2 and beta 3 subunits while mAb bd 24 is selective for the alpha 1 subunit of human and bovine, but not of rat origin. The latter antibody reacts with the rat alpha 1 subunit carrying an engineered Leu at position four, documenting the first epitope mapping of a GABAA receptor subunit-specific mAb. In contrast to mAbs bd 17 and bd 24, mAb bd 28 reacts with all GABAA receptor subunits tested but not with a glycine receptor subunit, suggesting the presence of shared epitopes on subunits of GABA-gated chloride channels.

MeSH Terms
Amino Acid Sequence Antibodies, Monoclonal/immunology Base Sequence Cell Line, Transformed Epitopes/immunology Humans Immunohistochemistry Kidney/cytology,embryology,ultrastructure Molecular Sequence Data Receptors, GABA-A/analysis,immunology,metabolism
Chemicals
Antibodies, Monoclonal Epitopes Receptors, GABA-A
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Ewert M
Center for Molecular Biology, University of Heidelberg, FRG.
Shivers B D
Lüddens H
Möhler H
Seeburg P H
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Article Info
Journal
The Journal of cell biology
Abbr.
J Cell Biol
ISSN
0021-9525
DOI
10.1083/jcb.110.6.2043
Published
1990-06-00
Pages
2043-8
Language
English
Region
United States
NLM ID
0375356
PMCID
PMC2116146
Subset
IM
External Links
PubMed source DOI: 10.1083/jcb.110.6.2043 PMC Full Text View journal details
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