In vitro folding and oligomerization of a membrane protein. Transition of bacterial porin from random coil to native conformation.

Porin, a channel-forming protein spanning bacterial outer membranes, was denatured in 6 M guanidinium hydrochloride or, alternatively, in sodium dodecyl sulfate at 95 degrees C. Circular dichroism spectra revealed that this protein, which in its native state consist of beta-pleated sheets as the sole detectable secondary structure, is transformed into random coil configuration in the chaotropic agent, or into alpha-helical structure in the detergent. From either state, the mature protein refolds in presence of amphiphilic molecules, attaining full structural and functional competence. As structural criteria, the native trimeric state was assayed by analytical ultracentrifugation, gel electrophoresis in sodium dodecyl sulfate, protease resistance, and circular dichroism spectroscopy. Channel formation in planar lipid bilayers reveals that the refolded protein is also functionally competent. It is concluded that the information required for the complete folding of porin is contained within the primary sequence of the mature polypeptide. The study of rapid refolding clearly reveals that this process occurs in the time range of seconds and that preexisting bilayers are not a prerequisite.